In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity

• Published in: Bio-medical materials and engineering Vol. 34; no. 6; pp. 561 - 575
• Main Authors: Ordóñez-Chávez, Guadalupe Del Carmén, Rodríguez-Fuentes, Nayeli, Peñaloza-Cuevas, Ricardo, Cervantes-Uc, José Manuel, Alcántara-Quintana, Luz Eugenia, Maya-García, Ixchel Araceli, Herrera-Valencia, Virginia Aurora, Mendiburu-Zavala, Celia Elena
• Format: Journal Article
• Language: English
• Published: Netherlands IOS Press BV 01.01.2023
• Subjects: Bone growth; Calcification; Calorimetry; Cattle; Cell adhesion; Cell viability; Crosslinking; Dental pulp; Deoxyribonucleic acid; Detergents; Differential scanning calorimetry; DNA; Fourier transforms; Glutaraldehyde; Infrared analysis; Infrared spectroscopy; Mechanical properties; Oral cavity; Pericardium; Regeneration; Regeneration (physiology); Scaffolds; Scanning electron microscopy; Soft tissues; Stem cells; Tensile tests; Thermogravimetric analysis; guided bone regeneration; oral cavity; Bovine pericardium; dental pulp stem cells
• ISSN: 0959-2989; 1878-3619
• DOI: 10.3233/BME-230027

Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity. To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR. BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs). The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs. This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.