Immobilized invertase studies on glass–ceramic support from coal fly ashes

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Vol. 214; no. 1; pp. 91 - 96
• Main Authors: Albertini, A.V.P., Silva, J.L., Freire, V.N., Santos, R.P., Martins, J.L., Cavada, B.S., Cadena, P.G., Rolim Neto, P.J., Pimentel, M.C.B., Martínez, C.R., Porto, A.L.F., Lima Filho, J.L.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 01.01.2013; Elsevier
• Subjects: activation energy; Applied sciences; beta-fructofuranosidase; Chemical engineering; coal; Coal fly ashes; enzyme kinetics; Exact sciences and technology; Glass–ceramic support; hydrolysis; Immobilization; Invertase; proteins; sucrose; temperature; X-ray diffraction; Zinc sulfate; Immobilization; Zinc sulfate; Glass–ceramic support; Invertase; Coal fly ashes; Reuse; X ray diffraction; Hydrolysis; Coal; pH; Glass-ceramic support; Thermodynamic properties; Glass ceramics; Activation energy
• ISSN: 1385-8947; 1873-3212
• DOI: 10.1016/j.cej.2012.10.029

► The new support from coal fly ashes added with ZnSO4 to immobilize invertase. ► The support is important such as resistance mechanical and microbiological. ► The sucrose hydrolysis must not be in the optimum temperature. ► An economical protocol was established to prepare derivative for invert sugar. ► The support can be a viable alternative to reduce environmental pollution. Invertase was covalently immobilized on new coal fly ashes glass-ceramic support with zinc sulfate (GCSZn). The coupling process of proteins was demonstrated by X-ray diffraction (XRD). There was no change in the optimum pH (4.6) but optimum temperature increased from 55°C for free invertase to 60°C for immobilized derivative. The activation energy decreased after immobilization (37.31±3.40kJ/mol) in spite of free invertase (51.34±5.21kJ/mol). There was an improvement in the Michaelis–Menten constant for sucrose hydrolysis after immobilization being 15times lower compared to that for free invertase (0.30±0.01mmol). After ten reuses at 25±2°C, the immobilized invertase lost only 9% of initial activity, but at the optimum temperature (60°C), the activity decrease was about 70%, what it is economically feasible under energetic view point for industrial application.