The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility

• Published in: Cryobiology Vol. 23; no. 5; pp. 406 - 414
• Main Authors: Schmehl, M.K., Anderson, S.P., Vazquez, I.A., Graham, E.F.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.1986; Elsevier
• Subjects: Animals; BELIER; Biological and medical sciences; Cell Survival; CONGELACION; CONGELATION; CONSERVACION DEL SEMEN; CONSERVATION DU SPERME; cryopreservation; DECONGELATION; DESCONGELACION; Dialysis; FERTILIDAD; FERTILITE; FERTILITY; FREEZING; Fundamental and applied biological sciences. Psychology; Male; Mammalian male genital system; Morphology. Physiology; MORUECO; RAMS; SEMEN; Semen - cytology; SEMEN PRESERVATION; Sheep; Sperm Motility; Spermatozoa - cytology; SPERME; SUPERVIVENCIA; SURVIE; SURVIVAL; THAWING; Vertebrates: reproduction; Vertebrata; Spermatozoa; Mammalia; Cryopreservation; Fertility; Semen; Sheep; Artiodactyla; Seminal plasma; Technique; Ungulata; Result
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/0011-2240(86)90025-8

The effect of dialysis on extended ram semen prior to cryopreservation was studied. Techniques were developed to improve post-thaw recovery of dialyzed semen and a fertility trial was used to evaluate the viability of dialyzed and frozen semen. Dialysis prior to freezing was shown to increase post-thaw recovery of motile cells and percentage of cells passing through a Sephadex filter. Freezing semen in pellets on dry ice was superior to freezing in French straws. Pellets were thawed in an aluminum thaw block at 42 to 45 °C before insemination of progestagen-PMSG synchronized ewes. Double inseminations were made at 12-hr intervals. Natural service of synchronized ewes was also made at 12-hr intervals as a control. There was no significant difference ( P > 0.05) in fertility between naturally serviced ewes (44.4%) and ewes inseminated with frozen semen (44.7%).