Modulation of rat peripheral polymorphonuclear leukocyte response by nitric oxide and arginine

• Published in: Blood Vol. 84; no. 8; pp. 2741 - 2748
• Main Authors: Seth, P, Kumari, R, Dikshit, M, Srimal, R C
• Format: Journal Article
• Language: English
• Published: United States 15.10.1994
• Subjects: Animals; Arachidonic Acid - pharmacology; Arginine - analogs & derivatives; Arginine - pharmacology; Free Radicals; Hydroxyl Radical - metabolism; Luminescent Measurements; Luminol - pharmacology; Male; Malondialdehyde - metabolism; Muramidase - metabolism; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - drug effects; Neutrophils - physiology; Nitric Oxide - pharmacology; Nitroprusside - pharmacology; omega-N-Methylarginine; Peroxidase - metabolism; Rats; Rats, Sprague-Dawley; Superoxides - metabolism; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.v84.8.2741.2741

The effect of nitric oxide (NO) on the luminol-dependent chemiluminescence (LCL) response of rat polymorphonuclear leukocytes (PMNLs) was analyzed by using sodium nitroprusside (SNP), a NO donor, and L-arginine (L-arg), a NO precursor. A significant reduction in the LCL intensity was observed in presence of SNP (100 mumol/L) or L-arg (5 or 10 mmol/L) in arachidonic acid (AA) phorbol ester (PMA) and formyl-methionyl-leucyl-phenylalanine stimulated PMNLs. However, opsonized zymosan-induced LCL was not attenuated significantly. Reduction in hydroxyl radical and superoxide generation was also observed in SNP- or L-arg-pretreated cells. D-Arg (10 mmol/L) pretreatment did not inhibit PMNLs' LCL response. Furthermore, methylene blue (5 mumol/L) and L-NG-mono methyl-L-arginine (100 or 300 mumol/L) significantly attenuated the LCL response, as induced by various agonists. Cyclic GMP did not alter the reactive oxygen species generation from rat PMNLs. In addition, AA-induced release of myeloperoxidase, a marker of azurophilic granules, was found to be enhanced in L-arg- (10 mmol/L) pretreated PMNLs. The results suggest that NO inhibits free radical generation from rat PMNLs.