Overexpression of DRG2 suppresses the growth of Jurkat T cells but does not induce apoptosis

• Published in: Archives of biochemistry and biophysics Vol. 422; no. 2; pp. 137 - 144
• Main Authors: Ko, Myoung Seok, Lee, Unn Hwa, Kim, Sin Il, Kim, Hyo Jeong, Park, Jeong Jae, Cha, Seung Ju, Kim, Sung Bum, Song, Hebok, Chung, Dae Kyun, Han, In Seob, Kwack, KyuBum, Park, Jeong Woo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.02.2004
• Subjects: Annexin A5 - metabolism; Apoptosis - physiology; Cell Aggregation; Cell Count; Cell Division - physiology; Developmentally regulated GTP-binding protein; Flow Cytometry; Gene Expression; Gene Expression Profiling; Growth suppression; GTP-Binding Proteins - biosynthesis; GTP-Binding Proteins - genetics; GTP-Binding Proteins - physiology; Humans; Jurkat; Jurkat Cells; Oligonucleotide Array Sequence Analysis; Overexpression; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; T-Lymphocytes - cytology; T-Lymphocytes - metabolism; T-Lymphocytes - physiology; Growth suppression; Developmentally regulated GTP-binding protein; Overexpression; Jurkat
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2003.12.028

Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexpression of DRG2 suppressed the growth and the aggregation of Jurkat cells but did not induce apoptotic cell death. We used cDNA microarray analysis to examine the global changes in gene expression induced by an overexpression of DRG2. DNA array analyses identified genes that may suppress cell growth at a number of levels in multiple signaling cascades in Jurkat cells and also several prosurvival genes that may protect cells from apoptosis.