Establishment and Analysis of Biological Characteristics of a Human Duodenal Carcinoma Cell Line, WDC-1

• Published in: Japanese journal of clinical oncology Vol. 29; no. 12; pp. 596 - 599
• Main Authors: Tani, Masaji, Tanimura, Hiroshi, Terasawa, Hiroshi, Yamade, Naohisa, Shono, Yoshiharu, Yamaue, Hiroki
• Format: Journal Article
• Language: English
• Published: England Foundation for Promotion of Cancer Research 01.12.1999; Oxford Publishing Limited (England)
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Adenocarcinoma - secondary; adhesion molecule; Aged; Aged, 80 and over; Animals; CA-19-9 Antigen - metabolism; carcinoembryonic antigen; Carcinoembryonic Antigen - biosynthesis; duodenal carcinoma; Duodenal Neoplasms - metabolism; Duodenal Neoplasms - pathology; Female; Humans; Integrins - metabolism; Lymphatic Metastasis; Mice; Mice, Inbred BALB C; Mice, Nude; neoadjuvant chemotherapy; Neoplasm Transplantation; tumor cell line; Tumor Cells, Cultured; Vascular Cell Adhesion Molecule-1 - metabolism
• ISSN: 0368-2811; 1465-3621
• DOI: 10.1093/jjco/29.12.596

Background: Duodenal carcinoma is very rare and its culture cell lines have rarely been established. Methods: Tumor cells separated from a surgically resected primary tumor of duodenal carcinoma were put into culture. The patient was an 81-year-old female and had metastatic lymph nodes. We investigated the biological characteristics of the culture cells including in vitro cell kinetics, karyotype, expression of tumor markers and integrins and tumorigenicity and histology in nude mice. Results: A new cell line, designated WDC-1, was established. This duodenal carcinoma cell line proliferated in a monolayered sheet with a doubling time of 50 h. The histological findings of the xenograft in nude mice were similar to those of the primary tumor. WDC-1 cells produced carcinoembryonic antigen and expressed 1 integrin and very late antigen (VLA)-4d in vitro. Conclusions: A duodenal carcinoma cell line was established, which is rare and may contribute to progress in understanding the biological features of duodenal cancer.