Regulation of aquaporin mRNA expression in rat kidney by water intake

• Published in: Journal of the American Society of Nephrology Vol. 10; no. 4; pp. 696 - 703
• Main Authors: MURILLO-CARRETERO, M. I, ILUNDAIN, A. A, ECHEVARRIA, M
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 01.04.1999
• Subjects: Animals; Aquaporins - analysis; Aquaporins - genetics; Base Sequence; Biological and medical sciences; Blotting, Northern; Culture Techniques; Dehydration - blood; Dehydration - physiopathology; Dehydration - urine; Down-Regulation; Drinking; Fundamental and applied biological sciences. Psychology; Kidney Cortex - cytology; Kidney Cortex - physiopathology; Kidney Medulla - cytology; Kidney Medulla - physiopathology; Kidney Tubules, Collecting - physiopathology; Molecular Sequence Data; Polymerase Chain Reaction; Rats; Rats, Wistar; Reference Values; RNA, Messenger - analysis; Up-Regulation; Vertebrates: urinary system; Water-Electrolyte Balance - physiology; Animal model; Hydration; Rat; Rodentia; Experimental study; Gene expression; Kidney; Vertebrata; Regulation(control); Mammalia; Messenger RNA; Urinary system; Animal; Physiology; Collecting duct; Aquaporin
• ISSN: 1046-6673; 1533-3450
• DOI: 10.1681/ASN.V104696

Three aquaporins (AQP) are present in the membrane of the principal collecting duct cells. On the apical side, the levels of AQP2 protein are increased in response to both arginine vasopressin and water deprivation. However, whether this change parallels changes in the abundance of AQP3 and AQP4 in the basolateral membrane is less well known. This study evaluates the effect of either dehydration or water loading on the rat kidney mRNA expression of AQP2, AQP3, and AQP4. Poly(A+)RNA was prepared from renal cortex and medulla of control, water-deprived, well hydrated, and water-deprived rats treated with OPC31260, a V2 receptor antagonist. Northern blots were done and mRNA levels were quantified using a PhosphorImager system. Relative to control, water deprivation increased the expression of cortical AQP2, -3, and -4, whereas water loading decreased the cortical and medullar expression of AQP2, -3, and -4. Therefore, in addition to AQP2 and -3, AQP4 expression is also regulated by water intake. Treatment with OPC31260 (40 mg/kg of weight per d) inhibited up to 20 to 30% the upregulation of AQP-mRNA induced by water deprivation. Blood values of arginine vasopressin and aldosterone were significantly increased by water deprivation, whereas they were unchanged by water overloading. Taken together, these results indicate that renal AQP2, -3, and -4 expression is regulated in a coordinated manner. Simultaneous up- or downregulation of the three transcripts occurred upon either water deprivation or water loading of animals, respectively. However, the signaling mechanism for the two long-term adaptive processes may be different, and, in addition to arginine vasopressin, other factors may be involved in the transcriptional regulatory processes.