Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo

• Published in: Archives of biochemistry and biophysics Vol. 465; no. 2; pp. 437 - 442
• Main Authors: Liu, Hong Yan, Zheng, Guolu, Zhu, Hongfa, Woldegiorgis, Gebre
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2007
• Subjects: Animals; Carnitine O-Palmitoyltransferase - metabolism; CAT; Diabetes Mellitus, Experimental - metabolism; Dietary; Dietary Fats - metabolism; Fasting - metabolism; Female; Gene expression; Gene Expression Regulation, Enzymologic; Hormonal; Hormones - metabolism; M-CPTI; Male; Mice; Mice, Transgenic; Muscle, Skeletal - metabolism; Organ Specificity; Promoter; Regulation; Tissue Distribution; Transgenic mice; Promoter; CAT; Transgenic mice; Hormonal; Regulation; M-CPTI; Gene expression; Dietary
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2007.06.026

Transgenic mice carrying the human heart muscle carnitine palmitoyltransferase I (M-CPTI) gene fused to a CAT reporter gene were generated to study the regulation of M-CPTI gene expression. When the mice were fasted for 48 h, CAT activity and mRNA levels increased by more than 2-fold in heart and skeletal muscle, but not liver or kidney. In the diabetic transgenic mice, there was a 2- to 3-fold increase in CAT activity and CAT mRNA levels in heart and skeletal muscle which upon insulin administration reverted to that observed with the control insulin sufficient transgenic mice. Feeding a high fat diet increased CAT activity and mRNA levels by 2- to 4-fold in heart and skeletal muscle of the transgenic mice compared to the control transgenic mice on regular diet. Overall, the M-CPTI promoter was found to be necessary for the tissue-specific hormonal and dietary regulation of the gene expression.