The force-generation process in active muscle is strain sensitive and endothermic: a temperature-perturbation study

• Published in: Journal of experimental biology Vol. 220; no. Pt 24; pp. 4733 - 4742
• Main Authors: Ranatunga, K W, Offer, Gerald
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 15.12.2017
• Subjects: Animals; Biomechanical Phenomena; Calcium; Calcium ions; In Vitro Techniques; Isotonic Contraction; Male; Muscle Tonus; Muscles; Psoas muscle; Psoas Muscles - physiology; Psoas Muscles - ultrastructure; Rabbits; Roads & highways; Skin; Strain; Temperature; Temperature effects; Tension; Velocity; Temperature effects; Ramp shortening; Muscle force generation
• ISSN: 0022-0949; 1477-9145
• DOI: 10.1242/jeb.167197

In experiments on active muscle, we examined the tension decline and its temperature sensitivity at the onset of ramp shortening and at a range of velocities. A segment (∼1.5 mm long) of a skinned muscle fibre isolated from rabbit psoas muscle was held isometrically (sarcomere length ∼2.5 µm) at 8-9°C, maximally Ca -activated and a ramp shortening applied. The tension decline with a ramp shortening showed an early decrease of slope (the transition) followed by a slower decrease in slope (the transition) to the steady (isotonic) force. The tension level at the initial transition and the time to that transition decreased as the velocity was increased; the length change to this transition increased with shortening velocity to a steady value of ∼8 nm half-sarcomere A small, rapid, temperature jump (T-jump) (3-4°C, <0.2 ms) applied coincident with the onset of ramp shortening showed force enhancement by T-jump and changed the tension decline markedly. Analyses showed that the rate of T-jump-induced force rise increased linearly with increase of shortening velocity. These results provide crucial evidence that the strain-sensitive cross-bridge force generation, or a step closely coupled to it, is endothermic.