Immunodetection of UCP1 in rat thymocytes
Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This im...
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Published in | Biochemical Society transactions Vol. 32; no. Pt 6; p. 1066 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.12.2004
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Subjects | |
Online Access | Get more information |
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Summary: | Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This implies that rat thymocytes contain uncoupling protein 1. |
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ISSN: | 0300-5127 |
DOI: | 10.1042/bst0321066 |