Immunodetection of UCP1 in rat thymocytes

Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This im...

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Bibliographic Details
Published inBiochemical Society transactions Vol. 32; no. Pt 6; p. 1066
Main Authors Carroll, A M, Haines, L R, Pearson, T W, Brennan, C, Breen, E P, Porter, R K
Format Journal Article
LanguageEnglish
Published England 01.12.2004
Subjects
Animals
Carrier Proteins - analysis
Carrier Proteins - isolation & purification
Electrophoresis, Polyacrylamide Gel
Immunoassay
Ion Channels
Membrane Proteins - analysis
Membrane Proteins - isolation & purification
Mitochondrial Proteins
Rats
Rats, Wistar
Thymus Gland - metabolism
Uncoupling Protein 1
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Summary:Thymi were dissected from rats and connective tissue was removed. Mitochondria were purified from isolated thymocytes and immunoblot analysis was performed using an antibody specific for uncoupling protein 1, which detected a 32.5 kDa protein associated with mitochondria from the thymocytes. This implies that rat thymocytes contain uncoupling protein 1.
ISSN:0300-5127
DOI:10.1042/bst0321066