An ethnopharmacological approach to evaluate antiparasitic and health-promoting abilities of Pueraria tuberosa (Willd.) DC. in livestock

• Published in: PloS one Vol. 19; no. 7; p. e0305667
• Main Authors: Mandal, Suman Kalyan, Mukherjee, Niladri, Ray, Anindya Sundar, Hazra, Samik, Saha, Sathi, Das, Swetarka, Joardar, Nikhilesh, Saha, Saradindu, Sinha Babu, Santi Prasad, Rahaman, Chowdhury Habibur
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.07.2024
• Subjects: Analysis; Animal diseases; Animals; Anthelmintic agents; Anthelmintics - pharmacology; Antioxidants; Antiparasitic agents; Antiparasitic Agents - pharmacology; Biocompatibility; Chlorocebus aethiops; Cost analysis; Energy value; Ethnobotany; Ethnomedicine; Ethnopharmacology; Female; Flavonoids; Flowers & plants; Health promotion; Helminthiasis; Herbal medicine; Humans; In vitro methods and tests; In vivo methods and tests; India; Integrated approach; Liquid chromatography; Livestock; Livestock - parasitology; Male; Medicinal plants; Mice; Molecular docking; Molecular Docking Simulation; Names; Nutritive value; Parasites; Parasitic diseases; Phenols; Phytochemicals; Phytochemicals - analysis; Phytochemicals - chemistry; Phytochemicals - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Tubers - chemistry; Protein binding; Proteins; Pueraria - chemistry; Pueraria tuberosa; Questionnaires; Tetracycline; Tetracyclines; Toxicity; Toxicity testing; Vero Cells; Veterinary medicine; India; West Bengal India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0305667

In eastern India, the tubers of Pueraria tuberosa (Willd.) DC. are used by the ethnic communities for its wide range of medicinal and nutritional value, especially to rejuvenate livestock health and to treat helminthiasis. The study is aimed to evaluate the ethnoveterinary medicinal importance of P. tuberosa as anthelmintic, to verify its nontoxic nature and identify the most potent phytoconstituents aided by in silico molecular docking technique. Ethnomedicinal data collected from 185 informants were quantitatively analyzed employing eight quantitative indices to highlight the use diversity and most frequently used part of the plant. High scores of certain indices employed, such as Use Value (UV = 0.52), Fidelity Level (FL = 68.42%) and Tissue Importance Value (TIV = 1) clearly illustrate an ethnomedicinal lead regarding medico-nutritional benefits of the tuber part used against intestinal helminthic diseases of veterinary animals. Based on this ethno-guided lead, root tuber has been investigated for its chemical profiling by the estimation of total phenolics, flavonoids, tannins and alkaloids, along with HPLC and GC-MS analyses. Anthelmintic property was evaluated with the tuber extracts by in vitro studies on some helminths of livestock and poultry birds, and it showed promising results against the tested parasites namely Cotylophoron cotylophorum, Raillietina tetragona and Setaria cervi. Toxicity assessments of tuber extract through in vitro and in vivo methods were performed using Vero cells and BALB/c mice. Nontoxic nature of the studied tuber extract was observed even in higher experimental doses. Out of 12 phytocompounds identified by GC-MS analysis, one compound [Morphinan-4,5-epoxy-3,6-di-ol,6- (7-nitrobenzofurazan-4-yl) amino-] exhibited the best binding conformations in cost of the lowest binding energy values with six target proteins that include one anti-inflammatory, one antioxidant, and four anthelmintic proteins. The findings of our study are found very encouraging to evaluate this tuber drug furthermore intensively towards the development of anthelmintic veterinary medicine.