The revelation of expressing region in the processed ceruloplasmin gene in human genome by biocomputational and biochemical methods

• Published in: Biophysical chemistry Vol. 115; no. 2; pp. 247 - 250
• Main Authors: Platonova, N.A., Vasin, A.V., Klotchenko, S.A., Tsymbalenko, N.V., Puchkova, L.V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2005
• Subjects: Amino Acid Sequence; Cell Line, Tumor; Ceruloplasmin; Ceruloplasmin - chemistry; Ceruloplasmin - genetics; Ceruloplasmin pseudogene expression; Computational Biology; Computers; Genome, Human; Humans; Mitochondria; Models, Biological; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Pseudogenes - genetics; Sequence Alignment; Ceruloplasmin; Ceruloplasmin pseudogene expression; Mitochondria
• ISSN: 0301-4622; 1873-4200
• DOI: 10.1016/j.bpc.2004.12.014

Translation in all open reading frames (ORF) of human ceruloplasmin (Cp) pseudogene revealed the only translating sequence of 984 nucleotides. The amino acid sequence contains a signal peptide for mitochondrial protein import at N-terminus. The predicted protein without taking the signal peptide into consideration has 92% identity to the corresponding Cp fragment. It contains 20 amino acid substitutions, 8 of them are significant. There is His-X-His motif in the center of a molecule that is typical for copper containing oxidases. Potential copper-binding site appears as a result of the substitution P923H along human Cp sequence. Cp pseudogene transcription product was found in the cultured human cell lines HepG2 and HuTu 80 using RT-PCR strategy. Cp polypeptides with molecular weight of nearly 30 kDa were found in mitochondria of HuTu 80 cells. The possible biological role of mitochondrial Cp is under discussion.