Effect of Cu,Zn superoxide dismutase disruption mutation on replicative senescence in Saccharomyces cerevisiae

• Published in: FEMS microbiology letters Vol. 177; no. 2; pp. 199 - 204
• Main Authors: Barker, M. Gordon, Brimage, Lydia J.E., Smart, Katherine A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.1999; Blackwell; Oxford University Press
• Subjects: Ageing; Baking yeast; Biological and medical sciences; Carbon; Carbon source; Carbon sources; Cell Division; Culture Media; Disruption; Ethanol; Ethanol - metabolism; Fundamental and applied biological sciences. Psychology; Galactose; Gene expression; Glucose - metabolism; Glycerol; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Haploidy; Life span; Microbiology; Mutation; Mycology; Oxidation; Oxygen Consumption; Replicative senescence; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Senescence; Superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase-1; Yeast; Yeasts; Superoxide dismutase; Carbon source; Replicative senescence; Ageing; Fungi; Senescence; Yeast; Enzyme; Development; Ascomycetes; Oxidoreductases; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1999.tb13732.x

Abstract The role of oxidative damage in determining the replicative lifespan of Saccharomyces cerevisiae was investigated using a wild-type haploid laboratory yeast and a Cu,Zn superoxide dismutase (sod1) mutant derivative on glucose, ethanol, glycerol and galactose media. SOD1 expression was necessary to ensure longevity on all carbon sources tested. Whilst carbon source and SOD1 gene expression do influence yeast lifespan, the relationship between the two factors is complex.