SiMYB56 Confers Drought Stress Tolerance in Transgenic Rice by Regulating Lignin Biosynthesis and ABA Signaling Pathway
Foxtail millet ( Setaria italica ) originated in China and is generally cultivated in arid and barren soil. Through long-term harsh environmental selection, foxtail millet has acquired significant drought resistance. However, the molecular mechanism of foxtail millet drought resistance is still unkn...
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Published in | Frontiers in plant science Vol. 11; p. 785 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
18.06.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Foxtail millet (
Setaria italica
) originated in China and is generally cultivated in arid and barren soil. Through long-term harsh environmental selection, foxtail millet has acquired significant drought resistance. However, the molecular mechanism of foxtail millet drought resistance is still unknown. Here, we identified a drought-induced R2R3-MYB transcription factor
SiMYB56
in foxtail millet. Overexpression of
SiMYB56
significantly enhances tolerance to drought stress in transgenic rice plants at both the vegetative and the reproductive stage and has no adverse effect on its normal growth. Compared with wild-type controls,
SiMYB56
-overexpressing rice plants had lower MDA content and higher lignin content under drought conditions. Quantitative real-time PCR and Transcriptional activity assays demonstrated that
SiMYB56
could activate expression of lignin biosynthesis genes under drought conditions. Also, we found that overexpression of
SiMYB56
can led to ABA accumulation in the seeds transgenic rice plants. Further experiments showed that Overexpression of
SiMYB56
can upregulate the expression of ABA synthesis and response related genes under drought conditions. In conclusion,
SiMYB56
may enhance the drought resistance of transgenic rice plants by regulating lignin biosynthesis and ABA signaling pathway, making
SiMYB56
a candidate gene for drought resistance improvement in gramineous crops. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Manoj Prasad, National Institute of Plant Genome Research (NIPGR), India; Kazuo Nakashima, Japan International Research Center for Agricultural Sciences (JIRCAS), Japan Edited by: Vicent Arbona, Jaume I University, Spain This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2020.00785 |