HIV-1 genetic diversity in Western Brittany, France

• Published in: FEMS immunology and medical microbiology Vol. 34; no. 1; pp. 65 - 71
• Main Authors: Vallet, Sophie, Legrand-Quillien, Marie-Christine, Roger, Christine, Bellein, Veronique, Perfezou, Pascale, de Saint-Martin, Luc, Garre, Michel, Brun-Vezinet, Francoise, Picard, Bertrand
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2002; Blackwell; Oxford University Press
• Subjects: Base Sequence; Biological and medical sciences; Contamination; DNA, Viral - genetics; Epidemiology; Female; France - epidemiology; Fundamental and applied biological sciences. Psychology; Genes, env; Genetic diversity; Genetic Variation; Genetics; Genotype; HIV; HIV Infections - epidemiology; HIV Infections - virology; HIV-1 - classification; HIV-1 - genetics; HIV-1 - isolation & purification; Human immunodeficiency virus; Human immunodeficiency virus type 1; Humans; Immunoassays; Male; Microbiology; Phylogeny; Population studies; Serotyping; Subtype; V3 loop; Virology; Viruses; France; Europe; Human immunodeficiency virus type 1; V3 loop; Phylogeny; Subtype; Human; HIV-1 virus; Retroviridae; AIDS; Genetic diversity; Lentivirus; Infection; Virus; Viral disease; Molecular epidemiology; Human immunodeficiency virus; Clinical isolate
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2002.tb00604.x

Abstract We describe human immunodeficiency virus type 1 (HIV-1) diversity in Western Brittany, France, and trace the dissemination of HIV-1 non-B subtype infection. The strategy for HIV-1 subtyping used involved subtype specific enzyme immunoassays, heteroduplex mobility assays and phylogenetic analysis of the sequences of env encoding the V3 loop region. Samples were obtained from 567 patients: 465 (82%) were of subtype B and 66 (11.6%) were not (20 were subtype A, 11 subtype C, four subtype D, seven subtype F, five subtype G and 19 others with circulating recombinant forms: 4CRF01_AE, 11CRF02_AG, 1H, 3CRF11_cpx). These findings are consistent with other studies of French populations. There is an epidemiological correlation between subtype B and homosexual or heterosexual contamination in France and between non-B subtype and heterosexual contamination in Africa.