Effects of the hypo-osmotic swelling test on the outcome of intracytoplasmic sperm injection for patients with only nonmotile spermatozoa available for injection: a prospective randomized trial

• Published in: Fertility and sterility Vol. 75; no. 3; pp. 480 - 484
• Main Authors: El-Nour, Atif M, Al Mayman, Hend A, Jaroudi, Kamal A, Coskun, Serdar
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2001; Elsevier Science
• Subjects: Biological and medical sciences; Birth control; Embryo Implantation; Embryo, Mammalian - physiology; Female; Gynecology. Andrology. Obstetrics; Humans; Hypo-osmotic swelling test; Hypotonic Solutions; ICSI; immotile sperm; Infertility, Male - therapy; Male; Medical sciences; Pregnancy; Prospective Studies; Sodium Chloride; Sperm Injections, Intracytoplasmic; Sperm Motility; Spermatozoa - physiology; Sterility. Assisted procreation; Treatment Outcome; immotile sperm; Hypo-osmotic swelling test; ICSI; Pregnancy; Human; Embryonic development; Randomization; Spermatozoa; Motility; Swelling; Ovum implantation; Intracytoplasmic sperm injection; Assisted procreation; Germinal cell
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/S0015-0282(00)01762-3

Objective: Hypo-osmotic swelling test (HOST) has been shown to be an effective method for the selection of live sperm. On-going pregnancies were obtained by using HOST-selected sperm. The aim of this study was to evaluate the effect of using HOST-selected “live” sperm versus nonselected sperm on the outcome of intracytoplasmic sperm injection cycles when only nonmotile sperm were available for injection. Design: Prospective randomized study. Setting: Governmental tertiary care hospital. Patient(s): Thirty ICSI cycles with no motile sperm were included in this study. Intervention(s): For the HOST group, potentially live spermatozoa detected by hypo-osmotic reaction of the tail were injected into oocytes. For the No-HOST group, the sperm were randomly injected into the oocytes without checking the viability. Main Outcome Measure(s): The fertilization, cleavage, embryo quality, pregnancy, and implantation rates were assessed for the two groups. Result(s): Among 30 cycles, 15 fall into each group. Fertilization, cleavage rates, and the number of good quality embryos were similar between two groups. Conclusion(s): HOST-selected live spermatozoa can be safely used for intracytoplasmic sperm injection to establish pregnancies. There is a tendency for higher pregnancy and implantation rates to result, but it does not reach statistical significance.