Accurate mass measurement of synthetic analogues of prazosine by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

• Published in: Rapid communications in mass spectrometry Vol. 15; no. 9; pp. 665 - 669
• Main Authors: Andalò, Christian, Bocchini, Paola, Pozzi, Romina, Galletti, Guido C.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2001
• Subjects: Adrenergic alpha-Antagonists - analysis; Calibration; Prazosin - analogs & derivatives; Prazosin - analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/rcm.272

Matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOFMS) has been investigated as a tool for accurate determination of the molecular mass of synthetic analogues of prazosine, a molecule used for the treatment of hypertension. Samples were dissolved in methanol, mixed with mass calibration standards, and crystallised on the target with α‐cyano‐4‐hydroxycinnamic acid as matrix. Acquisition of spectra was rapidly completed in reflectron mode, allowing high resolution (6000–10000) and sensitive (about 1–10 pmol of sample on target) determination of the synthetic products. The results show the effectiveness of MALDI‐TOFMS for accurate mass determination of these fairly large molecules, which are otherwise difficult to analyse by other high‐resolution mass spectrometric techniques. Copyright © 2001 John Wiley & Sons, Ltd.