CML-T1: A Cell Line Derived From T-Lymphocyte Acute Phase of Chronic Myelogenous Leukemia

• Published in: Blood Vol. 74; no. 4; pp. 1381 - 1387
• Main Authors: Kuriyama, Kazutaka, Gale, Robert Peter, Tomonaga, Masao, Ikeda, Shuichi, Yao, Eiichi, Klisak, Ivana, Whelan, Kathy, Yakir, Hadas, Ichimaru, Michito, Sparkes, Robert S., Dreazen, Orna
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.09.1989; The Americain Society of Hematology
• Subjects: Adult; Animal cells; Biological and medical sciences; Biomarkers - analysis; Biomarkers, Tumor - analysis; Cell cultures. Hybridization. Fusion; Cell Line; Cell Transformation, Neoplastic - analysis; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - pathology; Female; Fundamental and applied biological sciences. Psychology; Gene Rearrangement, T-Lymphocyte; Human T-lymphotropic virus 1 - analysis; Humans; Karyotyping; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology; Leukemia-Lymphoma, Adult T-Cell - genetics; Leukemia-Lymphoma, Adult T-Cell - metabolism; Leukemia-Lymphoma, Adult T-Cell - pathology; Molecular and cellular biology; T-Lymphocytes - metabolism; T-Lymphocytes - pathology; Tumor Cells, Cultured - analysis; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - pathology; Human; Cell culture; Morphology; Chronic myelocytic leukemia; Established cell line; T-Lymphocyte; Cytochemistry; Karyotype; Tumor cell; Immunological method
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V74.4.1381.1381

Most data suggest that malignant transformation in chronic myelogenous leukemia (CML) occurs in hematopoietic stem cell that is the progenitor of myelopoiesis and of B but not T lymphopoiesis. We established a T-lymphoid cell line (CML-T1) from a person with Ph-chromosome-negative CML in acute phase. Evidence of its T-lymphocyte origin includes the pattern cytochemical reactivity, reactivity with anti-T-cell monoclonal antibodies (MoAbs), and rearrangement of the β-T-cell receptor (TCRB) gene. CML-T1 cells have features of type IV thymocytes. Cytogenetic analyses indicate a 47,XX, del(11), t(6;7)(q23;q24), + mar karyotype. CML-T1 cells exhibit molecular changes typical of CML, including translocation of the ABL proto-oncogene from chromosome 9 to 22. rearrangement of the BCR gene, and transcription of a chimeric BCR-ABL messenger RNA (mRNA). The ABL insertion on chromosome 22 appears interstitial, similar to other cases of Ph-chromosome-negative CML. These data clearly indicate that T cells can be involved in acute-phase CML. CML-T1 should be useful in studying this process as well as that underlying Ph-chromosome-negative CML.