A potential role for endothelin-1 in peripheral vascular disease

• Published in: Journal of cardiovascular pharmacology Vol. 36; no. 5 Suppl 1; p. S93
• Main Authors: Dashwood, M R, Jagroop, I A, Gorog, D A, Bagger, J P
• Format: Journal Article
• Language: English
• Published: United States 2000
• Subjects: Autoradiography; Endothelin-1 - analysis; Endothelin-1 - physiology; Humans; Immunohistochemistry; Peripheral Vascular Diseases - drug therapy; Peripheral Vascular Diseases - etiology; Receptor, Endothelin A; Receptor, Endothelin B; Receptors, Endothelin - physiology
• ISSN: 0160-2446
• DOI: 10.1097/00005344-200036001-00030

The distribution of endothelin-1 (ET-1) and its receptors (ET(A)/ET(B)) has been studied in segments of femoral artery obtained from patients undergoing operation for peripheral vascular disease (PVD) using a combination of immunohistochemistry and autoradiography. Both receptor subtypes were located on the tunica media of vessel segments, with ET(A)-receptors predominating. Densitometric analysis showed that there was no difference in receptor binding to proximal/distal arterial segments from PVD patients. High-resolution autoradiography identified ET(B) binding to vascular nerves and vasa vasorum, mainly in distal portions of femoral arteries. Immunoreactive ET-1 was also identified that was associated with the vasa vasorum.