Expression Cloning of a cDNA Encoding a Novel Human Hematopoietic Growth Factor: Human Homologue of Murine T-Cell Growth Factor P40

• Published in: Blood Vol. 74; no. 6; pp. 1880 - 1884
• Main Authors: Yang, Yu-Chung, Ricciardi, Susan, Ciarletta, Agnes, Calvetti, James, Kelleher, Kerry, Clark, Steven C.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.11.1989; The Americain Society of Hematology
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Biological Factors - genetics; Blotting, Northern; Cloning, Molecular; Cytokines; DNA - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Glycoproteins - genetics; Growth Substances - genetics; Hematopoiesis; Humans; Interleukin-9; Leukemia, Megakaryoblastic, Acute - pathology; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; RNA, Messenger - genetics; Tumor Cells, Cultured; Human; Cell proliferation; Cell culture; Nucleotide sequence; Leukemia; Homology; Malignant hemopathy; Gene expression; Northern blotting; Cell line; Complementary DNA; Hematopoiesis; Immunoblotting assay; Molecular cloning; Growth factor
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V74.6.1880.1880

We used functional expression cloning in mammalian cells to identify a cDNA clone encoding a hematopoietic growth factor that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07E. Analysis of the sequence of this cDNA revealed striking similarity to that of a recently reported novel murine growth factor for helper T-cell clones designated T-cell growth factor P40. The mRNA for the human P40 protein is expressed by several different human T-cell lines and by mitogen-stimulated peripheral blood lymphocytes. The recombinant protein displays substantial size heterogeneity typical of other glycoprotein cytokines. These properties plus the observation that this cytokine may well act within both the lymphoid and myeloid lineages warrant the designation of P40 as interleukin-9.