Taxonomic identification and antagonistic activity of Streptomyces luomodiensis sp. nov. against phytopathogenic fungi

• Published in: Frontiers in microbiology Vol. 15; p. 1402653
• Main Authors: Qi, Dengfeng, Liu, Qiao, Zou, Liangping, Zhang, Miaoyi, Li, Kai, Zhao, Yankun, Chen, Yufeng, Feng, Junting, Zhou, Dengbo, Wei, Yongzan, Wang, Wei, Zhang, Lu, Xie, Jianghui
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 27.05.2024
• Subjects: antagonistic mechanism; antifungal activity; banana Fusarium disease; Microbiology; novel species; Streptomyces; taxonomic identification; Streptomyces; antagonistic mechanism; banana Fusarium disease; taxonomic identification; antifungal activity; novel species
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2024.1402653

Banana wilt caused by f. sp. tropical race 4 (Foc TR4) is a devastating fungal disease. Biocontrol strategies hold immense potential for inhibiting the spread of Foc TR4. Here, 30 actinobacteria were isolated from soils and screened for their antagonistic activity against Foc TR4. Strain SCA4-21 was selected due to its strongest antagonistic activity against Foc TR4. Strain SCA4-21 also exhibited strong antagonistic activity against the other eight phytopathogenic fungi. The strain was identified as the genus according to its physiological, biochemical, and phenotypic characteristics. The phylogenetic trees of 16S rRNA sequences demonstrated that strain SCA4-21 formed a subclade with HM 35 and/or NRRL B-5491 with low bootstrap values. Considering that 16S rRNAs did not provide sufficient resolution for species-level identification, the whole genome of strain SCA4-21 was sequenced. Multilocus sequence analysis (MLSA) based on five housekeeping gene alleles ( , , , , and ) revealed that strain SCA4-21 clustered into subsp. NBRC 13472 with 100% of bootstrap value. The analysis of the genome-based phylogeny also approved the results. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) were 91.26 and 44.30%, respectively, with values below the respective species level threshold of 95 and 70%. Hence, strain SCA 4-21 represented a novel species within the genus , named sp. nov. The type strain is SCA4-21 (=GDMCC4.340  = JCM36555 ). By the CAZymes analysis, 348 carbohydrate-active enzymes (CAZymes) were detected, including 15 chitinases and eight β-1,3-glucanases. The fermentation broth of strain SCA4-21 , exhibiting strong antagonistic activity against Foc TR4, demonstrated high activities of chitinase and β-1,3-glucanase, which might be involved in antifungal activity. Our results showed an innovative potential biocontrol agent for managing plant fungal diseases, specifically banana wilt.