Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea
Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite dif...
Saved in:
Published in | Biochip journal Vol. 6; no. 4; pp. 325 - 334 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
The Korean BioChip Society (KBCS)
01.12.2012
Springer Nature B.V 한국바이오칩학회 |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21–23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species. |
---|---|
AbstractList | Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21–23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species. Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21-23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species. KCI Citation Count: 3 |
Author | Hwang, Seung Yong Lee, Sukchan Lee, Youn-Ho Yum, Seungshic Lee, Gunsup Woo, Seonock Moh, Sang Hyun Lee, Taek-Kyun Park, Heung-Sik Park, So Yun |
Author_xml | – sequence: 1 givenname: Gunsup surname: Lee fullname: Lee, Gunsup email: tklee@kiost.ac organization: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology – sequence: 2 givenname: So Yun surname: Park fullname: Park, So Yun organization: Medical Research Center of Neural Dysfunction, Gyeonsang National University – sequence: 3 givenname: Seungshic surname: Yum fullname: Yum, Seungshic organization: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology – sequence: 4 givenname: Seonock surname: Woo fullname: Woo, Seonock organization: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology – sequence: 5 givenname: Youn-Ho surname: Lee fullname: Lee, Youn-Ho organization: Marine Living Resources Research Department, Korea Institute of Ocean Science and Technology – sequence: 6 givenname: Seung Yong surname: Hwang fullname: Hwang, Seung Yong organization: Department of Biochemistry, Hanyang University & GenoCheck Co. Ltd – sequence: 7 givenname: Heung-Sik surname: Park fullname: Park, Heung-Sik organization: Korea South Pacific Ocean Research Center, Korea Institute of Ocean Science and Technology – sequence: 8 givenname: Sang Hyun surname: Moh fullname: Moh, Sang Hyun organization: Department of Genetic Engineering, Sungkyunkwan University – sequence: 9 givenname: Sukchan surname: Lee fullname: Lee, Sukchan organization: Department of Genetic Engineering, Sungkyunkwan University – sequence: 10 givenname: Taek-Kyun surname: Lee fullname: Lee, Taek-Kyun organization: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology |
BackLink | https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001719591$$DAccess content in National Research Foundation of Korea (NRF) |
BookMark | eNp1kU1r3DAQhkVJoZuPH9CboKcenM7ItmQfl3w1NDRQkrNQ5NFGiVfaSN5A_n3kupBT5zIw7_MOM7yH7CDEQIx9RThFAPUjYy1AVoCikg00FXxiKwG9qFTX1Adshb0qqujgCzvJ-QlK1TW2olsxf06vNMbdlsLEo-Pnv9fcPvoddzHxV0reeWsmH8MsipZvvU3RjBtD3MZxJDvRwF2KW57jfnqkFMrc5MmMPNFm9vnAf8VE5ph9dmbMdPKvH7H7y4u7s5_Vze3V9dn6prJ1C1PllOmH4cFKSYBmEDC0UkGLjcRWSjCqQ9tJh5YaJBhQ9QbLl2owg3Wi7esj9n3ZG5LTz9braPzfvon6Oen1n7trjdiBqqGw3xZ2l-LLnvKkn-I-hXKeFr0AlNj1M4ULVV7POZHTu-S3Jr1pBD0HoJcAdAlAzwHo2SMWTy5s2FD62Px_0zu9tIiC |
CitedBy_id | crossref_primary_10_1007_s13206_014_8306_9 crossref_primary_10_1016_j_scitotenv_2021_149755 crossref_primary_10_1007_s13206_014_8410_x |
Cites_doi | 10.1111/j.1471-8286.2007.01678.x 10.3732/ajb.93.8.1101 10.1111/j.1529-8817.2005.00136.x 10.1080/10635150500354886 10.1111/j.1755-0998.2008.02199.x 10.1073/pnas.0510466103 10.1098/rspb.2002.2218 10.1098/rsbl.2003.0025 10.1186/1476-069X-10-54 10.1007/s13206-011-5111-6 10.1186/1741-7007-5-24 10.1093/molbev/msm092 10.1023/B:VERC.0000014207.01900.5c 10.1111/j.1755-0998.2009.02633.x 10.20506/rst.20.2.1284 |
ContentType | Journal Article |
Copyright | The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2012 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2012. |
Copyright_xml | – notice: The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2012 – notice: The Korean BioChip Society and Springer-Verlag Berlin Heidelberg 2012. |
DBID | AAYXX CITATION 8FE 8FG 8FH AFKRA ARAPS AZQEC BBNVY BENPR BGLVJ BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P P5Z P62 PQEST PQQKQ PQUKI ACYCR |
DOI | 10.1007/s13206-012-6404-0 |
DatabaseName | CrossRef ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Central Advanced Technologies & Aerospace Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection ProQuest One Community College ProQuest Central ProQuest Central Student SciTech Premium Collection Biological Sciences Biological Science Database Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition Korean Citation Index |
DatabaseTitle | CrossRef Advanced Technologies & Aerospace Collection ProQuest Central Student Technology Collection ProQuest Biological Science Collection ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials ProQuest One Academic Eastern Edition SciTech Premium Collection ProQuest One Community College ProQuest Technology Collection ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central Advanced Technologies & Aerospace Database ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest One Academic |
DatabaseTitleList | Advanced Technologies & Aerospace Collection |
Database_xml | – sequence: 1 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 2092-7843 |
EndPage | 334 |
ExternalDocumentID | oai_kci_go_kr_ARTI_1180730 10_1007_s13206_012_6404_0 |
GroupedDBID | --- -EM 06D 0R~ 0VY 1N0 203 23N 2KG 2VQ 30V 4.4 406 408 40D 5GY 8TC 96X 9ZL AAAVM AAFGU AAHNG AAIAL AAJKR AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO AAZMS ABDZT ABECU ABFGW ABFTV ABHLI ABJNI ABJOX ABKAS ABKCH ABMQK ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABXPI ACBMV ACBRV ACBYP ACGFS ACHSB ACIGE ACIPQ ACKNC ACMDZ ACMLO ACOKC ACPRK ACTTH ACVWB ACWMK ADHHG ADHIR ADINQ ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEFTE AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETCA AEVLU AEVTX AEXYK AFLOW AFNRJ AFQWF AFWTZ AFZKB AGAYW AGDGC AGGBP AGJBK AGMZJ AGQMX AGWZB AGYKE AHAVH AHBYD AHKAY AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AKQUC ALFXC ALMA_UNASSIGNED_HOLDINGS AMKLP AMXSW AMYLF AMYQR ANMIH AOCGG ASPBG AVWKF AXYYD AYJHY AZFZN BGNMA CSCUP DDRTE DNIVK DPUIP DU5 EBLON EBS EIOEI EJD ESBYG FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FYJPI GGCAI GGRSB GJIRD GQ6 GQ7 HF~ HMJXF HRMNR HZ~ I0C IKXTQ IWAJR IXD J-C J0Z JBSCW JZLTJ KOV LLZTM M4Y NPVJJ NQJWS NU0 O9- O9J OK1 P9N PT4 R9I RLLFE RSV S1Z S27 S3B SCM SHX SISQX SNE SNPRN SNX SOHCF SOJ SPISZ SQXTU SRMVM SSLCW STPWE T13 TSG U2A UG4 UOJIU UTJUX UZXMN VC2 VFIZW W48 WK8 Z45 Z7X ZMTXR ~A9 AACDK AAJBT AASML AAYXX ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AFKRA AGQEE AGRTI AIGIU ARAPS BBNVY BENPR BGLVJ BHPHI CCPQU CITATION H13 HCIFZ M7P ROL SJYHP 8FE 8FG 8FH AAYZH AZQEC DWQXO GNUQQ LK8 P62 PQEST PQQKQ PQUKI ACYCR |
ID | FETCH-LOGICAL-c350t-f7a9ddbc66e01ad20d5670514615660a781c86f1ce41e0d179a18437dadcf2593 |
IEDL.DBID | AGYKE |
ISSN | 1976-0280 |
IngestDate | Tue Nov 21 21:11:17 EST 2023 Sat Oct 19 00:54:49 EDT 2024 Thu Sep 12 19:33:24 EDT 2024 Sat Dec 16 12:00:30 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Keywords | Mitochondrial cytochrome c oxidase subunit I Microalgae DNA chip assay Marker gene DNA barcoding |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c350t-f7a9ddbc66e01ad20d5670514615660a781c86f1ce41e0d179a18437dadcf2593 |
Notes | G704-SER000001574.2012.6.4.004 |
PQID | 2920161890 |
PQPubID | 2043955 |
PageCount | 10 |
ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_1180730 proquest_journals_2920161890 crossref_primary_10_1007_s13206_012_6404_0 springer_journals_10_1007_s13206_012_6404_0 |
PublicationCentury | 2000 |
PublicationDate | 2012-12-01 |
PublicationDateYYYYMMDD | 2012-12-01 |
PublicationDate_xml | – month: 12 year: 2012 text: 2012-12-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Heidelberg |
PublicationPlace_xml | – name: Heidelberg |
PublicationTitle | Biochip journal |
PublicationTitleAbbrev | BioChip J |
PublicationYear | 2012 |
Publisher | The Korean BioChip Society (KBCS) Springer Nature B.V 한국바이오칩학회 |
Publisher_xml | – name: The Korean BioChip Society (KBCS) – name: Springer Nature B.V – name: 한국바이오칩학회 |
References | Yoon, Kim, Jeong, Jung, Chung (CR6) 2008; 2 Moniz, Kaczmarska, Gravenor (CR12) 2009; 9 Moretti, Turchini, Bellagamba, Caprino (CR3) 2003; 27 Tamura, Dudley, Nei, Kumar (CR18) 2007; 24 Kim (CR8) 2011; 5 Hajibabaei, Singer, Clare, Hebert (CR14) 2007; 5 Cunningham, Meghen (CR4) 2001; 20 Hebert, Gregory (CR13) 2005; 54 Folmer, Black, Hoch, Lutz, Vrijenhoek (CR17) 1994; 3 Robba, Russell, Barker, Brodie (CR10) 2006; 93 Kim (CR1) 2008; 18 Hinder (CR2) 2011; 10 Alverson, Kolnick (CR11) 2005; 41 Hajibabaei, Janzen, Burns, Hallwachs, Hebert (CR16) 2006; 103 Engelmann (CR19) 2009; 9 Hebert, Ratnasingham, de Waard (CR9) 2003; 270 Hebert, Cywinska, Ball, DeWaard (CR15) 2003; 270 Ratnasingham, Hebert, Gravenor (CR5) 2007; 7 Park (CR7) 2010; 4 O. Folmer (6404_CR17) 1994; 3 H. Yoon (6404_CR6) 2008; 2 P.D.N. Hebert (6404_CR9) 2003; 270 L. Robba (6404_CR10) 2006; 93 M. Hajibabaei (6404_CR14) 2007; 5 E. Cunningham (6404_CR4) 2001; 20 S. Ratnasingham (6404_CR5) 2007; 7 J.C. Engelmann (6404_CR19) 2009; 9 V. Moretti (6404_CR3) 2003; 27 K. Tamura (6404_CR18) 2007; 24 D. Kim (6404_CR1) 2008; 18 S. Kim (6404_CR8) 2011; 5 A.J. Alverson (6404_CR11) 2005; 41 S.L. Hinder (6404_CR2) 2011; 10 J.Y. Park (6404_CR7) 2010; 4 M.B.J. Moniz (6404_CR12) 2009; 9 P.D. Hebert (6404_CR13) 2005; 54 P.D.N. Hebert (6404_CR15) 2003; 270 M. Hajibabaei (6404_CR16) 2006; 103 |
References_xml | – volume: 7 start-page: 355 year: 2007 end-page: 364 ident: CR5 article-title: BOLD: The Barcode of Life Data System publication-title: Mol. Ecol. Notes. doi: 10.1111/j.1471-8286.2007.01678.x contributor: fullname: Gravenor – volume: 3 start-page: 294 year: 1994 end-page: 299 ident: CR17 article-title: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates publication-title: Mol. Mar. Biol. Biotechnol. contributor: fullname: Vrijenhoek – volume: 18 start-page: 1621 year: 2008 end-page: 1629 ident: CR1 article-title: Red to red-the marine bacterium Hahella chejuensis and its product prodigiosin for mitigation of harmful algal blooms publication-title: J. Microbiol. Biotechnol. contributor: fullname: Kim – volume: 93 start-page: 1101 year: 2006 end-page: 1108 ident: CR10 article-title: Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta) publication-title: Am. J. Bot. doi: 10.3732/ajb.93.8.1101 contributor: fullname: Brodie – volume: 41 start-page: 1248 year: 2005 end-page: 1257 ident: CR11 article-title: Intragenomic Nucleotide Polymorphism Among Small Subunit (18S) Rdna Paralogs in the Diatom Genus Skeletonema (Bacillariophyta) 1 publication-title: J. Phycol. doi: 10.1111/j.1529-8817.2005.00136.x contributor: fullname: Kolnick – volume: 54 start-page: 852 year: 2005 end-page: 859 ident: CR13 article-title: The promise of DNA barcoding for taxonomy publication-title: Syst. Biol. doi: 10.1080/10635150500354886 contributor: fullname: Gregory – volume: 9 start-page: 83 year: 2009 end-page: 93 ident: CR19 article-title: Modelling cross hybridization on phylogenetic DNA microarrays increases the detection power of closely related species publication-title: Mol. Ecol. Resour. doi: 10.1111/j.1755-0998.2008.02199.x contributor: fullname: Engelmann – volume: 103 start-page: 968 year: 2006 end-page: 971 ident: CR16 article-title: DNA barcodes distinguish species of tropical Lepidoptera publication-title: Proc. Natl. Acad. Sci. U S A doi: 10.1073/pnas.0510466103 contributor: fullname: Hebert – volume: 270 start-page: 313 year: 2003 ident: CR15 article-title: Biological identifications through DNA barcodes publication-title: Proc. R. Soc. Lond., B, Biol. Sci. doi: 10.1098/rspb.2002.2218 contributor: fullname: DeWaard – volume: 270 start-page: S96 year: 2003 ident: CR9 article-title: Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species publication-title: Proc. R. Soc. Lond., B, Biol. Sci. doi: 10.1098/rsbl.2003.0025 contributor: fullname: de Waard – volume: 10 start-page: 54 year: 2011 ident: CR2 article-title: Toxic marine microalgae and shellfish poisoning in the British isles: history, review of epidemiology, and future implications publication-title: Environ. Health. doi: 10.1186/1476-069X-10-54 contributor: fullname: Hinder – volume: 5 start-page: 72 year: 2011 end-page: 77 ident: CR8 article-title: DNA chip for species identification of Korean freshwater fish: A case study publication-title: BioChip J. doi: 10.1007/s13206-011-5111-6 contributor: fullname: Kim – volume: 2 start-page: 287 year: 2008 end-page: 295 ident: CR6 article-title: Development of salmon identification DNA chip based on mitochondrial COIII-ND3-ND4L variations publication-title: Bio-Chip J. contributor: fullname: Chung – volume: 4 start-page: 197 year: 2010 end-page: 203 ident: CR7 article-title: A DNA microarray for species identification of cetacean animals in Korean water publication-title: Bio-Chip J. contributor: fullname: Park – volume: 5 start-page: 24 year: 2007 ident: CR14 article-title: Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring publication-title: BMC Biol. doi: 10.1186/1741-7007-5-24 contributor: fullname: Hebert – volume: 24 start-page: 1596 year: 2007 ident: CR18 article-title: MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0 publication-title: Mol. Biol. Evol. doi: 10.1093/molbev/msm092 contributor: fullname: Kumar – volume: 27 start-page: 497 year: 2003 end-page: 505 ident: CR3 article-title: Traceability issues in fishery and aquaculture products publication-title: Vet. Res. Commun. doi: 10.1023/B:VERC.0000014207.01900.5c contributor: fullname: Caprino – volume: 20 start-page: 491 year: 2001 ident: CR4 article-title: Biological identification systems: genetic markers publication-title: Rev. Sci. Tech. contributor: fullname: Meghen – volume: 9 start-page: 65 year: 2009 end-page: 74 ident: CR12 article-title: Barcoding diatoms: Is there a good marker? publication-title: Mol. Ecol. Resour. doi: 10.1111/j.1755-0998.2009.02633.x contributor: fullname: Gravenor – volume: 2 start-page: 287 year: 2008 ident: 6404_CR6 publication-title: Bio-Chip J. contributor: fullname: H. Yoon – volume: 7 start-page: 355 year: 2007 ident: 6404_CR5 publication-title: Mol. Ecol. Notes. doi: 10.1111/j.1471-8286.2007.01678.x contributor: fullname: S. Ratnasingham – volume: 10 start-page: 54 year: 2011 ident: 6404_CR2 publication-title: Environ. Health. doi: 10.1186/1476-069X-10-54 contributor: fullname: S.L. Hinder – volume: 54 start-page: 852 year: 2005 ident: 6404_CR13 publication-title: Syst. Biol. doi: 10.1080/10635150500354886 contributor: fullname: P.D. Hebert – volume: 9 start-page: 83 year: 2009 ident: 6404_CR19 publication-title: Mol. Ecol. Resour. doi: 10.1111/j.1755-0998.2008.02199.x contributor: fullname: J.C. Engelmann – volume: 4 start-page: 197 year: 2010 ident: 6404_CR7 publication-title: Bio-Chip J. contributor: fullname: J.Y. Park – volume: 93 start-page: 1101 year: 2006 ident: 6404_CR10 publication-title: Am. J. Bot. doi: 10.3732/ajb.93.8.1101 contributor: fullname: L. Robba – volume: 3 start-page: 294 year: 1994 ident: 6404_CR17 publication-title: Mol. Mar. Biol. Biotechnol. contributor: fullname: O. Folmer – volume: 5 start-page: 72 year: 2011 ident: 6404_CR8 publication-title: BioChip J. doi: 10.1007/s13206-011-5111-6 contributor: fullname: S. Kim – volume: 270 start-page: 313 year: 2003 ident: 6404_CR15 publication-title: Proc. R. Soc. Lond., B, Biol. Sci. doi: 10.1098/rspb.2002.2218 contributor: fullname: P.D.N. Hebert – volume: 9 start-page: 65 year: 2009 ident: 6404_CR12 publication-title: Mol. Ecol. Resour. doi: 10.1111/j.1755-0998.2009.02633.x contributor: fullname: M.B.J. Moniz – volume: 24 start-page: 1596 year: 2007 ident: 6404_CR18 publication-title: Mol. Biol. Evol. doi: 10.1093/molbev/msm092 contributor: fullname: K. Tamura – volume: 20 start-page: 491 year: 2001 ident: 6404_CR4 publication-title: Rev. Sci. Tech. doi: 10.20506/rst.20.2.1284 contributor: fullname: E. Cunningham – volume: 18 start-page: 1621 year: 2008 ident: 6404_CR1 publication-title: J. Microbiol. Biotechnol. contributor: fullname: D. Kim – volume: 27 start-page: 497 year: 2003 ident: 6404_CR3 publication-title: Vet. Res. Commun. doi: 10.1023/B:VERC.0000014207.01900.5c contributor: fullname: V. Moretti – volume: 41 start-page: 1248 year: 2005 ident: 6404_CR11 publication-title: J. Phycol. doi: 10.1111/j.1529-8817.2005.00136.x contributor: fullname: A.J. Alverson – volume: 103 start-page: 968 year: 2006 ident: 6404_CR16 publication-title: Proc. Natl. Acad. Sci. U S A doi: 10.1073/pnas.0510466103 contributor: fullname: M. Hajibabaei – volume: 270 start-page: S96 year: 2003 ident: 6404_CR9 publication-title: Proc. R. Soc. Lond., B, Biol. Sci. doi: 10.1098/rsbl.2003.0025 contributor: fullname: P.D.N. Hebert – volume: 5 start-page: 24 year: 2007 ident: 6404_CR14 publication-title: BMC Biol. doi: 10.1186/1741-7007-5-24 contributor: fullname: M. Hajibabaei |
SSID | ssj0000331528 |
Score | 1.93882 |
Snippet | Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as... |
SourceID | nrf proquest crossref springer |
SourceType | Open Website Aggregation Database Publisher |
StartPage | 325 |
SubjectTerms | Algae Aquatic microorganisms Biodiversity Biomedical Engineering and Bioengineering Biotechnology Chemistry Chemistry and Materials Science Coastal zone Cytochrome-c oxidase DNA barcoding DNA chips DNA microarrays Functional foods & nutraceuticals Gene sequencing Medical equipment Original Research Phylogeny Red tides Species Taxonomy 생물공학 |
SummonAdditionalLinks | – databaseName: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1JS8QwFA4uFy-iqFg3gnhSgmnTptOTjMvggoOIA95CmkWL2o4z4__3vU7rqKC9FBrawpeX977kbYQcxKKTxNI5lgmdsRgupm2iGZd57k0YausxOfm2Ly8H8fVj8tgcuI2bsMpWJ9aK2lYGz8iPsasSFnfP-MnwnWHXKPSuNi005sliFMbopl08vejf3X-dsnAhwEDV-XBgdxn6EVvXZp0_J6J6Qx0xGWM0xg_jNF-O_A_e-ctVWlug3gpZbqgj7U7nepXMuXKNFN-ifmjl6Xm_S81zMaRARimIKUYC1eDjYJTQNwzAw_wNR1EEQN05SzHHhI6rOti9hOcaKOMrxZ4N8F5R0psKqOU6GfQuHs4uWdM-gRmR8Anzqc6szY2UjgPmEbeJTLHcucQ9G9dpJzQd6UPj4tBxCytTY_OX1GprPOyKxAZZKKvSbRLqjXC5FB7omYjTPMxzmabI5VLuNKzpgBy2uKnhtEqGmtVDRpAVgKwQZMUDsg_IqhdTKKxtjfenSr2MFDD4K4Ul6UDrBGSnBV41q2qsZjIQkKN2MmbDf_5x6_-PbZOlCGWgDlLZIQuT0YfbBaoxyfcaefoEYSHOPA priority: 102 providerName: ProQuest |
Title | Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea |
URI | https://link.springer.com/article/10.1007/s13206-012-6404-0 https://www.proquest.com/docview/2920161890 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001719591 |
Volume | 6 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
ispartofPNX | BioChip Journal, 2012, 6(4), , pp.325-334 |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LTxsxEB6RcGgvpdBWpKXIqnpq5ci73vUmx5QSoKgRqhqJniw_IUq7i5Jw6a9nZpMlQMuBvViy96H1jMcznplvAD5mspdnKgTel6bPM7y48bnhQlkbXZIYHyk5-ftIHY-zb-f5-Qakt0cX5bTbeCRrQb3OdZNpbfymXGUUOdGCzZzwvtqwOTj6dbo-WRFS4qZU58DhXsvJd9i4M__3nnsbUqucxXu65gP3aL3rDLeWmYDzGqyQgk2m3euF7bq__0I5PuGHXsKLlRLKBkuu2YaNUO7As4Om9tsrmNyJJWJVZF9HA-YuJ1cMVVyGzE_xRTVJaTDN2R8K66OskMCIsVCIBs8oc4XNqzqEvsR-g4rob0aVIPC5SclOK1RYX8N4ePjz4JivijJwJ3Ox4LEwfe-tUyoIpGQqfK4KAlFXZAkKU_QS11MxcSFLgvC43g2VlCm88S6irSXfQLusyrALLDoZrJIRlT6ZFTaxVhUFaYiFCAYlRQc-NZTRV0vsDb1GWabZ0zh7mmZPiw58QNrpqZtoQsym9qLS05lGu-BEE9AdyrIO7DWk1au1OtdUr4vKBvRx-HNDqvXwo198-6S738HzlGhdR8LsQXsxuw7vUZ9Z2H1o9YZH-ys2xvbL4ejsB_aO08ENeobsVw |
link.rule.ids | 315,786,790,12792,21416,27955,27956,33406,33777,41114,41556,42183,42625,43633,43838,52144,52267 |
linkProvider | Springer Nature |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3JTsMwELVYDnBBIECU1UKcQBZOnDjtCVVAKVtPIHGzHC9QFZLSlv9nxk0oIEEukWIlkZ7HM8-ejZCjRDTTRDrHWkK3WAIX0zbVjMs89yaKtPWYnHzfk93H5OYpfaoO3MZVWGWtE4OitqXBM_JT7KqExd1b_Gz4zrBrFHpXqxYa82QxEVKgnDc7V19nLFwIME8hGw6sLkMvYu3YDNlzIg7b6ZjJBGMxfpim-WLkf7DOX47SYH86q2SlIo60PZ3pNTLninXS_xbzQ0tPL3ptal76QwpUlIKQYhxQgB4H45S-YfgdZm84igIAys5ZihkmdFyGUPcCnmsgjK8UOzbAe_2C3pZALDfIY-fy4bzLquYJzIiUT5jPdMva3EjpOCAec5vKDIudS9yxcZ01I9OUPjIuiRy3sC41tn7JrLbGw55IbJKFoizcFqHeCJdL4YGciSTLozyXWYZMLuNOw4pukOMaNzWc1shQs2rICLICkBWCrHiDHAKyamD6Citb4_25VIORAv5-rbAgHeicBtmtgVfVmhqrmQQ0yEk9GbPhP_-4_f_HDshS9-H-Tt1d9253yHKM8hDCVXbJwmT04faAdEzy_SBZn6MKz8M |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9NAEB7RVIJeyluEFlghTiC3a-96nRyjltASiDhQqZyWfUKUYkeJe-mv74xjK7SCA8IXS14_d3Z3vvF8MwPwRopBLlUIyVCYYSJxS4zPTcKVtdGlqfGRgpM_T9XJmfx4np-3dU5XHdu9c0muYxooS1NZHy58PNwEvomssYSzREmiUWzBtsRZK3uwPfrwbbL5zcKFQA3VBMSh4k3Ikdj5Nv90nxvaaatcxhvA85avtFFB4_vwvXv5NfNkfnBZ2wN3dSuv43983QPYbeEpG63H00O4E8pHcO-oqwr3GGa_sYxYFdnxdMTcz9mCIfhlOC2IedQImxqznP0iwh_FiwRGQw6X1-AZxbSwVdWQ60s8bhCiXjCqEYHXzUo2qRDKPoGz8fuvRydJW64hcSLndRILM_TeOqUCRxln3OeqoPTqimxEbopB6gYqpi7INHCPK4GhYjOFN95FtMLEU-iVVRmeAYtOBKtERDgoZGFTa1VREHYseDC4hvThbScmvVhn5dCb_MvUexp7T1Pvad6H1yhIPXczTbm0af-j0vOlRovhVFMKPFzl-rDfyVm3s3ilqZIXFRQYYvO7Tmyb5r8-8fk_nf0K7n45HutPp9PJHuxkJPaGLrMPvXp5GV4g6Knty3ZgXwOXuPWd |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Development+of+DNA+chip+for+verification+of+25+microalgae+collected+from+southern+coastal+region+in+Korea&rft.jtitle=Biochip+journal&rft.au=Lee%2C+Gunsup&rft.au=Park%2C+So+Yun&rft.au=Yum%2C+Seungshic&rft.au=Woo%2C+Seonock&rft.date=2012-12-01&rft.pub=The+Korean+BioChip+Society+%28KBCS%29&rft.issn=1976-0280&rft.eissn=2092-7843&rft.volume=6&rft.issue=4&rft.spage=325&rft.epage=334&rft_id=info:doi/10.1007%2Fs13206-012-6404-0&rft.externalDocID=10_1007_s13206_012_6404_0 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1976-0280&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1976-0280&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1976-0280&client=summon |