Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea

• Published in: Biochip journal Vol. 6; no. 4; pp. 325 - 334
• Main Authors: Lee, Gunsup, Park, So Yun, Yum, Seungshic, Woo, Seonock, Lee, Youn-Ho, Hwang, Seung Yong, Park, Heung-Sik, Moh, Sang Hyun, Lee, Sukchan, Lee, Taek-Kyun
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean BioChip Society (KBCS) 01.12.2012; Springer Nature B.V; 한국바이오칩학회
• Subjects: Algae; Aquatic microorganisms; Biodiversity; Biomedical Engineering and Bioengineering; Biotechnology; Chemistry; Chemistry and Materials Science; Coastal zone; Cytochrome-c oxidase; DNA barcoding; DNA chips; DNA microarrays; Functional foods & nutraceuticals; Gene sequencing; Medical equipment; Original Research; Phylogeny; Red tides; Species; Taxonomy; 생물공학; Mitochondrial cytochrome c oxidase subunit I; Microalgae; DNA chip assay; Marker gene; DNA barcoding
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-012-6404-0

Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21–23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species.