Effect of house dust mite on neutrophil apoptosis by cytokine secretion in lymphocytes

• Published in: Molecular & cellular toxicology Vol. 12; no. 4; pp. 371 - 379
• Main Authors: Lee, Na Rae, Lee, Ji-Sook, Baek, Seung Yeop, Kim, Da Hye, Gu, Ayoung, Kim, Seong Yeol, Lee, Soo Jin, Kim, In Sik
• Format: Journal Article
• Language: English
• Published: Incheon The Korean Society of Toxicogenomics and Toxicoproteomics 01.12.2016; Springer Nature B.V; 대한독성 유전단백체 학회
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Apoptosis; Biomedical and Life Sciences; Cell Biology; Cytokines; Granulocyte-macrophage colony-stimulating factor; Interleukin 6; Interleukin 8; Leukocytes (neutrophilic); Life Sciences; Lymphocytes; Monocyte chemoattractant protein 1; Neutrophils; Original Paper; Pharmacology/Toxicology; Phosphorylation; 생물학; Allergy; Mite; Neutrophils apoptosis; Lymphocytes; Cytokine
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-016-0041-y

Although extract of Dermatophagoides farinae (DF) alone had no effect on neutrophil apoptosis, it inhibited neutrophil apoptosis in neutrophils cocultured with lymphocytes in both normal and allergic subjects. DF showed a stronger inhibition on the apoptosis of allergic neutrophils cocultured with allergic lymphocytes than that of normal neutrophils cocultured with normal lymphocytes. DF induced the secretion of IL-6, IL-8, MCP-1, and GM-CSF of the normal and allergic lymphocytes. The cytokine secretion due to DF in allergic lymphocytes is higher than in normal lymphocytes. The cytokine secretion of normal and allergic lymphocytes induced by DF was suppressed by PAR2i, a PAR2 inhibitor, LY294002, a PI3K inhibitor, AKTi, an Akt inhibitor, PD98059, an ER K inhibitor, and BAY-11-7085, an NF-κB inhibitor. Phosphorylation of ERK induced by DF was suppressed by PAR2i, LY294002 and AKTi, and NF-κB activity due to DF was inhibited by PAR2i, LY294002, AKTi, and PD98059.