Application of Acoustic Microscopy for Visualization of Structural Displacement during the Development of Fish Embryos

• Published in: Journal of communications technology & electronics Vol. 67; no. 4; pp. 456 - 462
• Main Authors: Burlakov, A. B., Titov, S. A., Bogachenkov, A. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.04.2022; Springer; Springer Nature B.V
• Subjects: Acoustic microscopy; Applications of Radiotechnology and Electronics in Biology and Medicine; Cell division; Communications Engineering; Embryonic development; Embryos; Engineering; Frequency ranges; Networks; Structural members; Thickness; Ultrasonic imaging; Ultrasonic testing; Ultrasound imaging
• ISSN: 1064-2269; 1555-6557
• DOI: 10.1134/S1064226922040027

—A technique for non-invasive ultrasound imaging of the processes of embryonic development of small biological organisms has been developed. A scanning acoustic microscope with an operating frequency range of 50–100 MHz has been created, which makes it possible to record ultrasound data in time-lapse mode and observe in vivo transformation and movement of structural elements of the embryo. Using the developed technique, studies of the loach embryo ( Misgurnus fossilis ), which develops directly in the immersion cell of the microscope. For the 6th and 15th stages of embryo development, data records were made with a duration of 10 min, demonstrating the processes of cell division and movement. It has been shown that at the 15th stage, the movement of cells in the inner and outer regions of the cell layer with a thickness of 0.1 mm occurs in opposite directions at speeds of approximately 0.3 and 0.1 μm/s, respectively.