Silencing of long noncoding RNA XIST protects against renal interstitial fibrosis in diabetic nephropathy via microRNA-93-5p-mediated inhibition of CDKN1A

• Published in: American journal of physiology. Renal physiology Vol. 317; no. 5; pp. F1350 - F1358
• Main Authors: Yang, Jindou, Shen, Yan, Yang, Xia, Long, Yanjun, Chen, Shuang, Lin, Xin, Dong, Rong, Yuan, Jing
• Format: Journal Article
• Language: English
• Published: United States 01.11.2019
• Subjects: Animals; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Diabetes Mellitus, Experimental; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - pathology; Female; Gene Silencing; Humans; Male; Mice; Mice, Inbred C57BL; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Oligonucleotide Array Sequence Analysis; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; long noncoding RNA; X-inactive specific transcript; cyclin-dependent kinase inhibitor 1A; microRNA-93-5p; diabetic nephropathy; renal interstitial fibrosis
• ISSN: 1931-857X; 1522-1466
• DOI: 10.1152/ajprenal.00254.2019

Long noncoding RNAs (lncRNAs) have been reported to play an important role in diabetic nephropathy (DN). However, the molecular mechanism involved in this process remains poorly understood. Thus, the present study aimed to explore the function and molecular mechanism of dysregulated lncRNA X-inactive specific transcript (XIST) in DN. DN mouse models were established by streptozotocin treatment, and human renal tubular epithelial HK-2 cells were exposed to high glucose to produce an in vitro model. XIST was highly expressed in renal tissues of patients with DN, mice with DN, and high glucose-exposed HK-2 cells. To identify the interaction among XIST, miR-93-5p, and cyclin-dependent kinase inhibitor 1A (CDKN1A) and to analyze the functional significance of their interaction in renal interstitial fibrosis, we altered endogenous expression of XIST and miR-93-5p and CDKN1A. Dual-luciferase reporter assay results suggested that XIST was highly expressed in the kidney tissue of DN mice and high glucose-exposed HK-2 cells. XIST was identified to be a lncRNA that could bind to miR-93-5p, and CDKN1A was a target of miR-93-5p. Downregulated expression of XIST led to an increase in miR-93-5p expression, thereby decreasing CDKN1A and suppressing renal interstitial fibrosis in DN. Consistently, XIST knockdown reduced the expression of fibrosis markers (fibronectin, collagen type IV, and transforming growth factor-β ). Restoration of CDKN1A or decreasing miR-93-5p yielded a reversed effect on renal interstitial fibrosis. In conclusion, our study demonstrated that silenced XIST inducing miR-93-5p-dependent CDKN1A inhibition was beneficial for preventing renal interstitial fibrosis in DN, which may provide a future strategy to prevent the progression of DN.