Propagation and xanthone content of Gentianella austriaca shoot cultures

• Published in: Plant cell, tissue and organ culture Vol. 94; no. 3; pp. 329 - 335
• Main Authors: Vinterhalter, B., Janković, T., Šavikin, K., Nikolić, R., Vinterhalter, D.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2008; Springer
• Subjects: Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Gentianella; Life Sciences; Original Paper; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; In vitro propagation; Xanthones; Shoot cultures; Precocious flowering; Vegetals; Propagation; Flowering; Micropropagation; Shoot; Culture
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-008-9374-0

Shoot cultures of Gentianella austriaca (A. & J. Kerner) Dostal established from seedling epicotyls were maintained on MS medium supplemented with 2.22 μM BA and 0.54 μM NAA. A characteristic feature of these cultures was precocious flowering, which appeared in all rapidly elongating shoots. Flower development arrested shoot elongation and multiplication of shoot cultures. Continuous shoot propagation was possible only by use of small axillary or adventitious buds as explants for subculturing. Flowering could not be suppressed by GA 3 addition or by cultivation in short-day conditions. The highest rooting percentage (47.3% with 7.83 roots per explant) was achieved on media with 4.92 μM IBA. Shoot cultures contained the same types of secondary metabolites as plants from nature. Xanthones were the major constituents, with DMB (demethylbellidifolin), DGL (demethylbellidifolin-8- O -glucoside) and BGL (bellidifolin-8- O -glucoside) present at roughly two times lower concentrations than in samples from nature. Secondary metabolite production was strongly affected by the presence of BA in the medium.