An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent

• Published in: Analytical biochemistry Vol. 580; pp. 49 - 55
• Main Authors: Zhang, Jia-Tong, Kang, Tian-Shu, Wong, Suk-Yu, Pei, Ren-Jun, Ma, Dik-Lung, Leung, Chung-Hang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2019
• Subjects: Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; G-quadruplex; G-Quadruplexes; Herbal plant extracts; Iridium - chemistry; Luminescence; Ochratoxins - analysis; OTA; TRES; TRES; Herbal plant extracts; OTA; G-quadruplex
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2019.06.005

A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing. •A G-quadruplex-based platform has been developed for the monitoring of OTA in herbal plant extracts samples.•This platform exhibits promising advantages including easy operation, low-cost and no complicated pre-processing required.•This platform allows the probe signal to be separated from intense background fluorescence.•The simple platform displays good sensitivity for OTA via time-resolved emission spectroscopy (TRES).