Isomer-specific effect of conjugated linoleic acid on inflammatory adipokines associated with fat accumulation in 3T3-L1 adipocytes

• Published in: Journal of medicinal food Vol. 9; no. 3; pp. 307 - 312
• Main Authors: Ahn, I.S, Choi, B.H, Ha, J.H, Byun, J.M, Shin, H.G, Park, K.Y, Do, M.S
• Format: Journal Article
• Language: English
• Published: United States 01.09.2006
• Subjects: 3T3-L1 Cells; adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; adiponectin; Adiponectin - secretion; Animals; Apoptosis - drug effects; biomarkers; C-reactive protein; C-Reactive Protein - genetics; chemical structure; conjugated linoleic acid; cultured cells; cytokines; Cytokines - genetics; fat accumulation; gene expression; Gene Expression - drug effects; in vitro studies; inflammation; interleukin-6; Interleukin-6 - genetics; isomers; leptin; Leptin - secretion; linoleic acid; Linoleic Acids, Conjugated - pharmacology; Lipid Metabolism - drug effects; messenger RNA; Mice; RNA, Messenger - analysis; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - genetics
• ISSN: 1096-620X; 1557-7600
• DOI: 10.1089/jmf.2006.9.307

The purpose of this study was to examine the isomer-specific effect of conjugated linoleic acid (CLA) on inflammatory markers associated with fat accumulation in cultures of differentiating 3T3-L1 adipocytes. trans-10,cis-12 CLA (t10c12 CLA) reduced leptin secretion and fat accumulation. Linoleic acid (LA) and cis-9,trans-11 CLA (c9t11 CLA) increased them, but not significantly. t10c12 CLA and LA showed similar effects on mRNA expression of inflammatory markers. t10c12 CLA and LA tended to up-regulate the mRNA levels of inflammatory cytokines such as interleukin (IL)-6 (not significantly), tumor necrosis factor (TNF)-alpha, and C-reactive protein (CRP) with no significant change in the secretion of adiponectin, an anti-inflammatory adipokine. However, c9t11 CLA induced no significant change in the mRNA expression of IL-6, TNF-alpha, or CRP, but significantly increased adiponectin secretion. In conclusion, CLA exerted isomer-specific effects on fat accumulation and mRNA expression of inflammatory markers in 3T3-L1 adipocytes. t10c12 CLA up-regulated inflammatory markers in spite of the decreased fat accumulation, and TNF-alpha might be one of the causal factors.