Identification of Plasmodium GAPDH epitopes for generation of antibodies that inhibit malaria infection

sporozoite liver infection is an essential step for parasite development in its mammalian host. Previously, we used a phage display library to identify mimotope peptides that bind to Kupffer cells and competitively inhibit sporozoite-Kupffer cell interaction. These peptides led to the identification...

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Published inLife science alliance Vol. 1; no. 5; p. e201800111
Main Authors Cha, Sung-Jae, McLean, Kyle Jarrod, Jacobs-Lorena, Marcelo
Format Journal Article
LanguageEnglish
Published United States Life Science Alliance LLC 01.10.2018
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Summary:sporozoite liver infection is an essential step for parasite development in its mammalian host. Previously, we used a phage display library to identify mimotope peptides that bind to Kupffer cells and competitively inhibit sporozoite-Kupffer cell interaction. These peptides led to the identification of a Kupffer cell receptor-CD68-and a sporozoite ligand-GAPDH-that are required for sporozoite traversal of Kupffer cells and subsequent infection of hepatocytes. Here, we report that the C-terminal end of GAPDH interacts with the Kupffer CD68 receptor, and identify two epitopes within this region as candidate antigens for the development of antibodies that inhibit infection.
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ISSN:2575-1077
2575-1077
DOI:10.26508/lsa.201800111