Heterologous expression of two Physcomitrella patens group 3 late embryogenesis abundant protein (LEA3) genes confers salinity tolerance in arabidopsis
Salinity stress is a major limiting factor in agriculture and adversely affecting the whole plant. As a halophyte, the moss Physcomitrella patens , has been suggested to be an ideal model plant to study salinity tolerance and adaption. Two abiotic stress-responsive Group 3 Late Embryogenesis Abundan...
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Published in | Journal of plant biology = Singmul Hakhoe chi Vol. 59; no. 2; pp. 182 - 193 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.04.2016
Springer Nature B.V 한국식물학회 |
Subjects | |
Online Access | Get full text |
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Summary: | Salinity stress is a major limiting factor in agriculture and adversely affecting the whole plant. As a halophyte, the moss
Physcomitrella patens
, has been suggested to be an ideal model plant to study salinity tolerance and adaption. Two abiotic stress-responsive Group 3 Late Embryogenesis Abundant protein genes had been identified in
P. patens
and named as
PpLEA3-1
and
PpLEA3-2
, respectively. Functions of these two genes were analyzed by heterologous expressions in Arabidopsis, driven either by their native P. patens promoters or by the 35S CaMV constitutive promoter. Phenotype analysis revealed that p
LEA3
::
LEA3
, p
LEA3
::
LEA3
::GFP and 35S::
LEA3
::GFP transgenic lines had stronger salinity resistance than that in the wild type and empty-vector control. Further analysis showed that the contents of proline and soluble sugar were increased and the malondialdehyde (MDA) were repressed in these transgenic plants after exposure to salinity stress. Our observations indicate that these two Group 3
PpLEA
genes played a role in the adaption to salinity stress. |
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ISSN: | 1226-9239 1867-0725 |
DOI: | 10.1007/s12374-016-0565-7 |