A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences

The polymerase chain reaction (PCR) system allows the amplification of DNA segments between two regions of known sequence. The authors describe a procedure that extends this technique to sequences that lie outside the boundaries of known sequences. The approach simply requires inversion of the seque...

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Bibliographic Details
Published inNucleic acids research Vol. 16; no. 16; p. 8186
Main Authors TRIGLIA, T, PETERSON, M. G, KEMP, D. J
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.08.1988
Subjects
Animals
Antigens, Protozoan - genetics
Base Sequence
Biological and medical sciences
Biotechnology
Cloning, Molecular - methods
Fundamental and applied biological sciences. Psychology
Gene Amplification
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
Plasmodium falciparum - genetics
Amplification
Primer extension method
Nucleotide sequence
DNA
Genetic engineering
Method
Flanking sequence
Molecular cloning
In vitro
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Summary:The polymerase chain reaction (PCR) system allows the amplification of DNA segments between two regions of known sequence. The authors describe a procedure that extends this technique to sequences that lie outside the boundaries of known sequences. The approach simply requires inversion of the sequence of interest by circularization and re-opening at a different site.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.16.8186