A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences

The polymerase chain reaction (PCR) system allows the amplification of DNA segments between two regions of known sequence. The authors describe a procedure that extends this technique to sequences that lie outside the boundaries of known sequences. The approach simply requires inversion of the seque...

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Bibliographic Details
Published inNucleic acids research Vol. 16; no. 16; p. 8186
Main Authors TRIGLIA, T, PETERSON, M. G, KEMP, D. J
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.08.1988
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Summary:The polymerase chain reaction (PCR) system allows the amplification of DNA segments between two regions of known sequence. The authors describe a procedure that extends this technique to sequences that lie outside the boundaries of known sequences. The approach simply requires inversion of the sequence of interest by circularization and re-opening at a different site.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.16.8186