Differentiated response mechanisms of soil microbial communities to nitrogen deposition driven by tree species variations in subtropical planted forests

• Published in: Frontiers in microbiology Vol. 16; p. 1534028
• Main Authors: Hou, Zheng, Chen, Wen, Zhang, Xiaohua, Zhang, Donghui, Xing, Jinmei, Ba, Yong, Yu, Jie, Wang, Keqin, Zhang, Ya, Song, Yali
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12.03.2025
• Subjects: co-occurrence network; community structure; Microbiology; nitrogen deposition; planted forest; richness; soil microbial diversity; richness; soil microbial diversity; community structure; planted forest; co-occurrence network; nitrogen deposition
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2025.1534028

The increasing rate of atmospheric nitrogen deposition has severely affected the structure and function of these ecosystems. Although nitrogen deposition is increasing globally, the responses of soil microbial communities in subtropical planted forests remain inadequately studied. In this study, a four-year experimental simulation was conducted to assess the impacts of varying nitrogen deposition levels (CK: 0 g·N·m ·a ; N10: 10 g·N·m ·a ; N20: 20 g·N·m ·a ; N25: 25 g·N·m ·a ) on two subtropical tree species, Franch. and Franch. High-throughput sequencing was performed using the Illumina MiSeq platform. Statistical analyses, including analysis of variance (ANOVA), linear mixed-effects models, principal coordinate analysis (PCoA), analysis of similarity (ANOSIM), redundancy analysis (RDA), random forest analysis, and structural equation modeling (SEM), were used to examine the short-term responses of soil nutrients, bacterial communities, and fungal community structures to nitrogen deposition. The results showed that species differences led to variations in soil properties between the two forests, particularly a significant increase in soil pH in Franch. forests and a significant decrease in soil pH in Franch. forests. Nitrogen addition did not significantly affect microbial diversity in either Franch. or Franch. soils; however, forest type differences had a significant impact on bacterial diversity. The nitrogen addition significantly affected the relative abundance of specific microbial communities in both forest types, particularly altering the fungal community structure in the Franch forests, while no significant changes were observed in the bacterial community structure in either forest type. Furthermore, nitrogen addition increased the network complexity of bacterial communities in Franch. forests while decreasing network complexity in Franch. forests. Structural equation modeling indicated that nitrogen addition regulates soil bacterial and fungal diversity in both forest types by modifying nitrogen availability. These findings provide insights into the potential long-term impacts of nitrogen deposition on subtropical planted forest ecosystems and offer a theoretical basis for sustainable forest management and regulatory practices.