Nucleoli formation under inhibited RNA synthesis

Formation and fusion of nucleoli after mitosis were studied in cultures of Chinese hamster cells and meristematic cells of Allium cepa and A. fistulosum under blocked RNA synthesis. To identify precisely which cells had passed through mitosis under actinomycin D blockade, cell cultures with micronuc...

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Bibliographic Details
Published inExperimental cell research Vol. 93; no. 2; pp. 458 - 467
Main Authors Semeshin, V.F., Sherudilo, A.I., Belyaeva, E.S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1975
Subjects
Caffeine - pharmacology
Cell Line
Cell Nucleolus - drug effects
Cell Nucleolus - physiology
Cell Nucleolus - ultrastructure
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Dactinomycin - pharmacology
Diploidy
Dose-Response Relationship, Drug
Mitosis
onions
RNA - biosynthesis
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Summary:Formation and fusion of nucleoli after mitosis were studied in cultures of Chinese hamster cells and meristematic cells of Allium cepa and A. fistulosum under blocked RNA synthesis. To identify precisely which cells had passed through mitosis under actinomycin D blockade, cell cultures with micronuclei (induced by colchicine treatment), and binuclear Allium cells (induced by caffeine treatment), were used. It was found that in cells which have passed through mitosis after inhibition of RNA synthesis, the formation and fusion of nucleoli proceed more slowly than in cells not treated with actinomycin D; however, nucleoli appear and coalesce. Thus, telophase reconstruction of the nucleolus does not require simultaneous RNA synthesis and occurs at the expense of RNA that has been synthesized prior to mitosis.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(75)90472-3