Alkanesulfonate degradation by novel strains of Achromobacter xylosoxidans, Tsukamurella wratislaviensis and Rhodococcus sp., and evidence for an ethanesulfonate monooxygenase in A. xylosoxidans strain AE4

• Published in: Archives of microbiology Vol. 176; no. 6; pp. 406 - 414
• Main Authors: ERDLENBRUCH, Barbara N. S, KELLY, Donovan P, MURRELL, J. Colin
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.12.2001; Berlin
• Subjects: Achromobacter xylosoxidans; Actinomycetales - growth & development; Actinomycetales - metabolism; Alcaligenes - enzymology; Alcaligenes - growth & development; alkanesulfonates; Alkanesulfonates - metabolism; Alkanesulfonic Acids - metabolism; Biodegradation of pollutants; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Environment and pollution; ethanesulfonate monooxygenase; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; isethionic acid; Isolation and description; Microbiology; Mission oriented research; Oxidation-Reduction; Oxygenases - metabolism; Rhodococcus - growth & development; Rhodococcus - metabolism; Species Specificity; Sulfites - metabolism; taurine; Tsukamurella wratislaviensis; Taurine; Short chain; Diversity; Rhodococcus; Metabolism; Carbon; Uptake; Alcaligenes denitrificans subsp. xyloxidans; Strain; Actinomycetales; Degradation; Hydrolysis; Gordona; Absorption; Aminoacid; Achromobacter; Bacteria; Isolate; Isolation; Actinomycetes; Tsukamurella
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s002030100340

Novel isolates of Achromobacter xylosoxidans, Tsukamurella wratislaviensis and a Rhodococcus sp. are described. These grew with short-chain alkanesulfonates as their sole source of carbon and energy. T. wratislaviensis strain SB2 grew well with C(3)-C(6) linear alkanesulfonates, isethionate and taurine, Rhodococcus sp. strain CB1 used C(3)-C(10) linear alkanesulfonates, taurine and cysteate, but neither strain grew with ethanesulfonate. In contrast, A. xylosoxidans strain AE4 grew well with ethanesulfonate, making it the first bacterium to be described which can grow with this compound. It also grew with unsubstituted C(3)-C(5) alkanesulfonates and isethionate. Hydrolysis was excluded as a mechanism for alkanesulfonate metabolism in these strains; and evidence is given for a diversity of uptake and desulfonatase systems. We provide evidence for an initial monooxygenase-dependent desulfonation in the metabolism of ethanesulfonate and propanesulfonate by A. xylosoxidans strain AE4.