Can time-lapse culture combined with artificial intelligence improve ongoing pregnancy rates in fresh transfer cycles of single cleavage stage embryos?

• Published in: Frontiers in endocrinology (Lausanne) Vol. 15; p. 1449035
• Main Authors: Wang, Xiao, Wei, Qipeng, Huang, Weiyu, Yin, Lanlan, Ma, Tianzhong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 29.08.2024
• Subjects: Adult; Artificial Intelligence; Cleavage Stage, Ovum - cytology; Cleavage Stage, Ovum - physiology; Embryo Culture Techniques - methods; Embryo Implantation; Embryo Transfer - methods; Embryonic Development - physiology; Endocrinology; Female; Fertilization in Vitro - methods; fresh cycle; Humans; iDAscores; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Retrospective Studies; single cleavage stage embryo transfer; time-lapse culture; Time-Lapse Imaging; time-lapse culture; iDAscores; fresh cycle; artificial intelligence; single cleavage stage embryo transfer
• ISSN: 1664-2392; 1664-2392
• DOI: 10.3389/fendo.2024.1449035

With the rapid advancement of time-lapse culture and artificial intelligence (AI) technologies for embryo screening, pregnancy rates in assisted reproductive technology (ART) have significantly improved. However, clinical pregnancy rates in fresh cycles remain dependent on the number and type of embryos transferred. The selection of embryos with the highest implantation potential is critical for embryologists and influences transfer strategies in fertility centers. The superiority of AI over traditional morphological scoring for ranking cleavage-stage embryos based on their implantation potential remains controversial. This retrospective study analyzed 105 fresh embryo transfer cycles at the Centre for Reproductive Medicine from August 2023 to March 2024, following IVF/ICSI treatment at the cleavage stage. All embryos were cultured using time-lapse technology and scored using an automated AI model (iDAScore V2.0). Embryos were categorized into three groups based on the iDAScore V2.0: Group A (8 cells, iDA: 1.0-5.7); Group B (8 cells, iDA: 5.8-8.0); and Group C (>8 cells, iDA: 5.8-8.0). Clinical treatment outcomes, embryonic development, and pregnancy outcomes were analyzed and compared across the groups. Baseline characteristics such as patient age, AMH levels, AFC, and basal sex hormones showed no significant differences among the three groups (p > 0.05). The iDAscores were significantly higher in Group C (7.3 ± 0.5) compared to Group B (6.7 ± 0.5) and the iDAscores were significantly higher in Group B (6.7 ± 0.5) compared to Group A (4.8 ± 1.0) (p < 0.001).The mean number of high-quality embryos was highest in Group C (4.7 ± 3.0), followed by Group B (3.6 ± 1.7) and Group A (2.1 ± 1.2) (p < 0.001). There was no statistical difference (p = 0.392) in the ongoing pregnancy rate for single cleavage-stage transfers between Group B (54.5%, 30/55) and Group A (38.1%, 8/21), although there was a tendency for Group B to be higher. Combining time-lapse culture with AI scoring may enhance ongoing pregnancy rates in single cleavage-stage fresh transfer cycles.