Proteins derived from Prunus armeniaca kernel are possible to cause Immunoglobulin E reactivity in human sera

Allergic diseases are always caused by protein molecules and in most cases arise in individuals with documented immunologic reactions to foods, pollen, or plant IgE. However, proteins derived from herbal medicine which act as an allergen have not been widely studied. Here, we aim to confirm if the h...

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Published inMolecular & cellular toxicology Vol. 13; no. 2; pp. 213 - 220
Main Authors Kim, Kang-Hoon, Park, Ji Young, Lee, In-Seung, Kim, Yumi, Jang, Hyeung-Jin
Format Journal Article
LanguageEnglish
Published Incheon The Korean Society of Toxicogenomics and Toxicoproteomics 01.06.2017
Springer Nature B.V
대한독성 유전단백체 학회
Subjects
Allergens
Allergic diseases
Biomedical and Life Sciences
Cell Biology
Enzyme immunoassay
Food plants
Gel electrophoresis
Herbal medicine
Hypersensitivity
Immunoglobulin E
Immunoreactivity
Life Sciences
Medicinal plants
Original Paper
Peanuts
Pharmacology/Toxicology
Proteins
Sodium lauryl sulfate
Western blotting
생물학
Herbal medicine
Immunoglobulin E
Allergy-Q test
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Summary:Allergic diseases are always caused by protein molecules and in most cases arise in individuals with documented immunologic reactions to foods, pollen, or plant IgE. However, proteins derived from herbal medicine which act as an allergen have not been widely studied. Here, we aim to confirm if the herbal medicine Prunus armeniaca kernel at the proteomic level triggers IgE immunoreactivity. If it does, we can hypothesize what components of protein molecules in the herbal medicine Prunus armeniaca kernel react with and bind to IgE. To test this hypothesis, we utilized proteomic tools and SDS-PAGE stained with Coomassie blue and identified proteins using LC-MS/MS. We also assessed the degree of plasma IgE reactivity in an isolated protein of Prunus armeniaca kernel using western blots of human IgE and an enzyme immunoassay panel, the Allergy-Q test, in 400 human sera. As a result, we found three sera which were positive candidates as Prunus armeniaca kernel IgE binding proteins. The results imply that Prunus armeniaca kernel can act as an allergen given the identification of IgE binding proteins among the isolated proteins of Prunus armeniaca kernel.
ISSN:1738-642X
2092-8467
DOI:10.1007/s13273-017-0023-8