Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy

Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD)...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 56; no. 93; pp. 14669 - 14672
Main Authors Williams, Lara, Tischlik, Sonja, Scherer, Andreas, Fischer, Jörg Wolfram Anselm, Drescher, Malte
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 04.12.2020
Subjects
Chemical compounds
Chemical synthesis
Distance measurement
Electron paramagnetic resonance
Electron spin
Fluorescence
Labels
Magnetic dipoles
Proteins
Spectroscopy
Spectrum analysis
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Summary:Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD) spectroscopy in proteins that lack an intrinsic photoexcitable center. We report the first example of EPR distance determination using photoexcitable spin labels site-selectively attached via click or thiol chemistry.
Bibliography:Electronic supplementary information (ESI) available: Theoretical description and simulations of LaserIMD, label synthesis and chemical characterization, cw and transient EPR, triplet relaxation, EDFS spectra, TRX protein expression, purification and labeling, LaserIMD and 4-pulse DEER distance measurements. See DOI
10.1039/d0cc03101a
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc03101a