miR-182-5p Inhibits NKAPL Expression and Promotes the Proliferation of Osteosarcoma
Purpose Osteosarcoma, a malignant bone tumor, has the lowest survival rate among all pediatric cancers. NF-κB-activating protein-like (NKAPL) is highly homologous with NKAP. The expression of NKAPL is downregulated in primary liver cancer and breast cancer, and plays a role of tumor suppressor gene....
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Published in | Biotechnology and bioprocess engineering Vol. 26; no. 5; pp. 758 - 766 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Seoul
The Korean Society for Biotechnology and Bioengineering
01.10.2021
Springer Nature B.V 한국생물공학회 |
Subjects | |
Online Access | Get full text |
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Summary: | Purpose
Osteosarcoma, a malignant bone tumor, has the lowest survival rate among all pediatric cancers. NF-κB-activating protein-like (NKAPL) is highly homologous with NKAP. The expression of NKAPL is downregulated in primary liver cancer and breast cancer, and plays a role of tumor suppressor gene. However, the role of NKAPL in osteosarcoma has not been reported.
Materials and Methods
We explored the effect of NKAPL on the proliferation of osteosarcoma cells by immunohistochemical, RT-PCR, Western blot, and double luciferase reporter gene analysis.
Results
The low expression of NKAPL mRNA was correlated with distant metastasis (P = 0.017), tumor size (P = 0.023), and clinical stage (P < 0.001). The NKAPL expression level in MG63 and U2OS cells was lower than that in Nhost cells. Downregulation of NKAPL expression in Nhost cells could promote cell proliferation and upregulation of NKAPL expression in MG63 and U2OS cells could inhibit cell proliferation. miR-182-5p expression was negatively correlated with NKAPL mRNA expression (R
2
= 0.1169, P = 0.0099). After upregulating NKAPL expression, the Notch1, hes1, hey2, and cyclin D1 expression levels were significantly decreased, with G0/G1 phase arrest and G2/M phase reduction.
Conclusions
miR-182-5p targeted NKAPL and inhibit NKAPL expression in osteosarcoma. miR-182-5p could regulate cell cycle and promote tumor proliferation through upregulating Notch signaling pathway. |
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ISSN: | 1226-8372 1976-3816 |
DOI: | 10.1007/s12257-021-0019-z |