Visualization of enzymatic DNA extension by surface plasmon resonance imaging

The enzymatic extension of single-stranded DNA on a gold substrate was studied with surface plasmon resonance (SPR) imaging and atomic force microscopy. Extension of the DNA strands was carried out by TdT, which catalyzes the addition of different deoxynucleotidyl triphosphates (dNTPs) to the 3′-hyd...

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Bibliographic Details
Published inBiochip journal Vol. 5; no. 4; pp. 304 - 308
Main Authors Jung, Jaeyeon, Hyun, Jinho
Format Journal Article
LanguageEnglish
Published Heidelberg The Korean BioChip Society (KBCS) 01.12.2011
Springer Nature B.V
한국바이오칩학회
Subjects
Atomic force microscopy
Biomedical Engineering and Bioengineering
Biotechnology
Chemistry
Chemistry and Materials Science
DNA nucleotidylexotransferase
Original Research
Single-stranded DNA
Strands
Substrates
Surface plasmon resonance
생물공학
Surface plasmon resonance (SPR)
Microbead
DNA
Imaging
Enzymatic extension
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Summary:The enzymatic extension of single-stranded DNA on a gold substrate was studied with surface plasmon resonance (SPR) imaging and atomic force microscopy. Extension of the DNA strands was carried out by TdT, which catalyzes the addition of different deoxynucleotidyl triphosphates (dNTPs) to the 3′-hydroxyl end of DNA. The extension rate of the DNA strands depended on the dNTPs, in the order of dATP ≈dTTP>dCTP>dGTP. The time-dependence of the DNA extension was imaged using SPR.
Bibliography:G704-SER000001574.2011.5.4.010
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-011-5403-x