Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR

An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli, a multiplex polymerase chain reaction (PCR) encoding Shiga toxins (stx1 and stx2), intimin (eaeA), and ETT2 (...

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Published inJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 12; no. 3; pp. 157 - 159
Main Authors Osawa, Kayo, Shibata, Mariko, Nishiyama, Yoko, Kataoka, Nobumasa, Kurokawa, Manabu, Yamamoto, Go, Kinoshita, Shohiro
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.06.2006
Subjects
Diarrhea - microbiology
Diarrheagenic Escherichia coli
Enterohemorrhagic Escherichia coli
Escherichia coli
Escherichia coli O157 - genetics
Escherichia coli O157 - isolation & purification
ETT2
Genes, Bacterial
Humans
Multiplex PCR
Polymerase Chain Reaction - methods
Multiplex PCR
Enterohemorrhagic Escherichia coli
Diarrheagenic Escherichia coli
ETT2
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Summary:An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli, a multiplex polymerase chain reaction (PCR) encoding Shiga toxins (stx1 and stx2), intimin (eaeA), and ETT2 (etrA) was developed for rapid detection. The ETT2 locus was identified not only in Shiga toxin-producing E. coli (STEC) but also in various non-STEC.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1341-321X
1437-7780
DOI:10.1007/s10156-006-0435-1