Hyperoxia Improves Oxygen Consumption in the Detached Feline Retina

• Published in: Investigative ophthalmology & visual science Vol. 48; no. 3; pp. 1335 - 1341
• Main Authors: Wang, Shufan, Linsenmeier, Robert A
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.03.2007; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Biological and medical sciences; Cats; Cell Survival; Eye and associated structures. Visual pathways and centers. Vision; Fundamental and applied biological sciences. Psychology; Hyperoxia - metabolism; Ion-Selective Electrodes; Oxygen - metabolism; Oxygen Consumption - physiology; Partial Pressure; Photoreceptor Cells, Vertebrate - metabolism; Retina - metabolism; Retinal Detachment - metabolism; Vertebrates: nervous system and sense organs; Oxygen consumption; Retina; Ophthalmology; Hyperoxia
• ISSN: 0146-0404; 1552-5783; 1552-5783
• DOI: 10.1167/iovs.06-0842

To investigate the effects of hyperoxia on retinal oxygenation and oxygen consumption in the detached feline retina. Retinal detachment was created in nine intact anesthetized cats by injecting 0.25% sodium hyaluronate in balanced salt solution into the subretinal space. Oxygen microelectrodes were used to collect spatial profiles of retinal Po(2) in both the attached and detached retina. A diffusion model was fitted to quantify photoreceptor oxygen consumption (Q(av)). In the detached retina, the Po(2) at the border between the retina and the fluid layer under the retina decreased; hyperoxia increased it to a level that was not significantly different from the control (attached retina, air breathing). Detachment did not change the Po(2) at the border between the avascular and vascularized retina; hyperoxia significantly increased the level. Oxygen consumption decreased to 47% +/- 18% of the control value in the detached retina during normoxia; hyperoxia increased Q(av) to 68% +/- 17% of control. Hyperoxia increased the average inner retinal Po(2) (P(IR)) in the detached retina to a level higher than that during normoxia. Detachment did not change P(IR) during normoxia. Hyperoxia has been shown to improve photoreceptor survival in the detached retina. The present work suggests that hyperoxia is protective because it allowed increased photoreceptor oxygen consumption. Whereas normal Po(2)s were maintained at the inner and outer border of the avascular region during hyperoxia, Q(av) was not restored to normal, suggesting that other factors are involved in photoreceptor dysfunction during detachment in addition to insufficient oxygen delivery.