In Vitro Response of Pepper Anther Culture (Capsicumannuum L.) Depending on Genotype, Nutrient Medium and Duration of Cultivation

The in vitro response of anther cultures of 19 Bulgarian pepper (Capsicum annuum L.) genotypes among which eight lines, seven varieties and four hybrids was examined using two induction media (C and Cm) within two time intervals for cultivation-12 and 40 days respectively, and two regeneration media...

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Published inBiotechnology, biotechnological equipment Vol. 25; no. 4; pp. 2604 - 2609
Main Authors Irikova, Teodora, Grozeva, Stanislava, Popov, Petar, Rodeva, Velichka, Todorovska, Elena
Format Journal Article
LanguageEnglish
Published Sofia Taylor & Francis 01.11.2011
Taylor & Francis Ltd
Subjects
Agricultural biotechnology
anther culture
Anthers
Capsicum annuum
Cultivation
Culture media
direct embryogenesis
duration of cultivation
Embryogenesis
Embryonic growth stage
Embryos
Flowers & plants
Genotype & phenotype
Genotypes
Hybrids
induction medium
Inoculation
Nutrient requirements
Nutrients
pepper (Capsicum annuum L.)
Peppers
Plantlets
Regeneration
Spices
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Summary:The in vitro response of anther cultures of 19 Bulgarian pepper (Capsicum annuum L.) genotypes among which eight lines, seven varieties and four hybrids was examined using two induction media (C and Cm) within two time intervals for cultivation-12 and 40 days respectively, and two regeneration media (R and Rm). Six lines, six varieties and three hybrids produced direct embryos in the cultivated anthers 35-40 days after inoculation in vitro. Regenerated plants were obtained from four lines, six varieties and one hybrid. It was established that 12 days of cultivation on medium C were not sufficient to initiate embryo formation in the tested genotypes while the longer one (40 days) revealed their ability for direct embryogenesis. Considerable percentage of embryos developed into plant-regenerants after being transferred on R medium (50-100%). Higher frequency of embryogenic anthers was recorded after 12 days of culturing on induction medium Cm but after their transfer on Rm medium the embryos did not develop into plantlets. These results suggest that the donor genotypes have specific requirements for nutrient medium and duration of induction cultivation.
Bibliography:ObjectType-Article-1
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content type line 23
ISSN:1310-2818
1314-3530
DOI:10.5504/BBEQ.2011.0090