A DNA microarray for species identification of cetacean animals in Korean water

Some whales caught incidentally or illegally in Korean waters have been introduced into the seafood market. Thus, the Korea Coast Guard (KCG) monitors and reports incidentally caught whales. However, species identification of some carcasses based on morphological features is difficult and often inac...

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Bibliographic Details
Published inBiochip journal Vol. 4; no. 3; pp. 197 - 203
Main Authors Park, Jung Youn, Kim, Ji-Hoon, An, Yong-Rock, Kim, Mi-Jung, Lee, Won Sun, An, Cheul Min, Jung, Jin-Wook, Kang, Jung-Ha, Moon, Hyo-Bang, Hwang, Seung Yong
Format Journal Article
LanguageEnglish
Published Heidelberg The Korean BioChip Society (KBCS) 01.09.2010
Springer Nature B.V
한국바이오칩학회
Subjects
Animals
Biomedical Engineering and Bioengineering
Biotechnology
Cetacea
Chemistry
Chemistry and Materials Science
Coastguard services
Cytochrome-c oxidase
Deoxyribonucleic acid
DNA
DNA chips
DNA microarrays
DNA probes
Dyes
Fluorescent dyes
Fluorescent indicators
Genetic diversity
Hybridization
Nucleotide sequence
Nucleotides
Oligonucleotides
Original Research
Seafood
Sequence analysis
Species
Whales
생물공학
Cetacean animals
DNA microarray system
Whale
Species identification
Cytochrome c oxidase subunit I
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ISSN1976-0280
2092-7843
DOI10.1007/s13206-010-4306-6

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Summary:Some whales caught incidentally or illegally in Korean waters have been introduced into the seafood market. Thus, the Korea Coast Guard (KCG) monitors and reports incidentally caught whales. However, species identification of some carcasses based on morphological features is difficult and often inaccurate, particular for processed whale products in the marketplace. For these reasons, we developed a DNA microarray that distinguishes 9 species of cetacean animals for quick and simple species identification. We verified genetic variations in whales and designed species-specific oligonucleotide probes by sequence analysis of mitochondrial cytochrome c oxidase subunit I. Fluorescent dye-labeled DNA targets were generated with a fluorescent dye-labeled primer and hybridized onto a DNA microarray consisting of short probes that were 29-37 nucleotides in length. A DNA microarray system using species-specific probes was successful at rapidly identifying 9 species of whales on the basis of hybridization patterns.
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G704-SER000001574.2010.4.3.005
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-010-4306-6