Sensitivity of Streptococcus thermophilus to chemical permeabilization

Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for beta-galactosidase activity. Na-deoxycholate and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their corresponding glyco-...

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Published inCurrent microbiology Vol. 32; no. 2
Main Authors Somkuti, G.A. (USDA, ARS, Eastern Regional Research Center, Philadelphia.), Dominiecki, M.E, Steinberg, D.H
Format Journal Article
LanguageEnglish
Published 01.02.1996
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BETA GALACTOSIDASA
BETA GALACTOSIDASE
BIOMASA
BIOMASSE
HIDROLISIS
HYDROLYSE
LACTOSA
LACTOSE
SALES BILIARES
SEL BILIAIRE
STREPTOCOCCUS THERMOPHILUS
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Summary:Streptococcus thermophilus cultures were treated with conjugated and unconjugated bile salts and tested for beta-galactosidase activity. Na-deoxycholate and chenodeoxycholate were more efficient permeabilizing agents than cholate, and all three bile salts were superior to their corresponding glyco- and tauro-conjugates. Treatment with sodium dodecyl sulfate resulted in the highest measurable beta-galactosidase levels in permeabilized cells, whereas response to Triton X-100 was variable and strain dependent. Na-deoxycholate, chenodeoxycholate, and sodium dodecyl sulfate caused cell injury and arrested culture growth for 4 h or longer. The nongrowing permeabilized biomass of S. thermophilus was used to hydrolyze lactose in aqueous solutions and milk
Bibliography:9612008
Q02
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900018