Gene expression profile of NFκB repressing factor (NKRF) knockdown cells by microarray analysis

• Published in: Biochip journal Vol. 6; no. 3; pp. 247 - 253
• Main Authors: Sun, Yaqiong, Zheng, Dan, Gu, Shaohua, Mao, Yumin, Xie, Yi
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean BioChip Society (KBCS) 01.09.2012; Springer Nature B.V; 한국바이오칩학회
• Subjects: Apoptosis; Binding sites; Biomedical Engineering and Bioengineering; Biotechnology; Cell cycle; Cell migration; Cell proliferation; Chemistry; Chemistry and Materials Science; DNA microarrays; DNA repair; Down-regulation; Gene expression; Gene regulation; Genes; HIV; Human immunodeficiency virus; NF-κB protein; Optical fibers; Original Research; RNA-mediated interference; Signal transduction; 생물공학; RNAi; Microarray; NKRF; Gene expression profile
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-012-6307-0

Human NFB repressing factor (NKRF) is a negative regulation transcription factor, which is able to repress transcription by binding to the negative regulatory element (NRE) near the NFκB binding site in certain genes’ promoters. Current researches reveals that NKRF represses the activation of IFN-, IL-8, hiNOS and HIV-1 by NFκB. We used optical fiber beadchip to analysis the different gene expression patterns of RNAi mediated NKRF knockdown HEK293 cells and found that several genes showed significant change of expression levels. Real-time PCR was performed to verify the changes of expression of candidate genes. We analyzed the function of candidate genes by searching the gene ontology databases and publications and revealed that these genes functioned in cell cycle, cell proliferation, apoptosis, cell migration, DNA repair, transcription, metabolism, response to stimulus and signal transduction. This study provides new perspectives on NKRF’s potential multiple functions.