Association between polymorphisms of the APOBEC3G gene and chronic hepatitis B viral infection and hepatitis B virus-related hepatocellular carcinoma

• Published in: World journal of gastroenterology : WJG Vol. 23; no. 2; pp. 232 - 241
• Main Authors: He, Xiu-Ting, Xu, Hong-Qin, Wang, Xiao-Mei, He, Xiu-Shu, Niu, Jun-Qi, Gao, Pu-Jun
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Inc 14.01.2017
• Subjects: Adult; APOBEC-3G Deaminase - genetics; Asian Continental Ancestry Group - genetics; Basic Study; Carcinoma, Hepatocellular - epidemiology; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - virology; Case-Control Studies; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Haplotypes; Hepatitis B virus - isolation & purification; Hepatitis B, Chronic - complications; Hepatitis B, Chronic - epidemiology; Hepatitis B, Chronic - genetics; Hepatitis B, Chronic - virology; Humans; Incidence; Liver Neoplasms - epidemiology; Liver Neoplasms - genetics; Liver Neoplasms - virology; Male; Mass Spectrometry; Middle Aged; Polymorphism, Single Nucleotide; Retrospective Studies; APOBEC3s; Progression; Hepatocellular carcinoma; Hepatitis B viral; Polymorphism
• ISSN: 1007-9327; 2219-2840; 2219-2840
• DOI: 10.3748/wjg.v23.i2.232

To determine the relationship between five gene single nucleotide polymorphisms and the incidence of hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). This association study was designed as a retrospective study, including 657 patients with chronic HBV infection (CHB) and 299 healthy controls. All subjects were ethnic Han Chinese. Chronic HBV-infected patients recruited between 2012 and 2015 at The First Hospital of Jilin University (Changchun) were further classified into HBV-related HCC patients ( = 287) and non-HCC patients ( = 370). Frequency matching by age and sex was performed for each group. Human genomic DNA was extracted from whole blood. Gene polymorphisms were identified using a mass spectroscopic method. There were no significant differences between the genotype and allele frequencies of the rs7291971, rs5757465 and rs5757463 gene polymorphisms, and risk of CHB and HBV-related HCC. The AG genotype and G allele for rs8177832 were significantly related to a decreased risk of CHB (OR = 0.67, 95%CI: 0.47-0.96; OR = 0.69, 95%CI: 0.50-0.95, respectively) and HCC (OR = 0.53, 95%CI: 0.34-0.84; OR = 0.58, 95%CI: 0.39-0.87, respectively). A significant relationship was found between rs2011861 computed tomography, TT genotypes and increased risk of HCC (OR = 1.69, 95%CI: 1.02-2.80; OR = 1.82, 95%CI: 1.08-3.06, respectively). Haplotype analyses showed three protective and four risk haplotypes for HCC. Also, one protective haplotype was found against CHB. This study indicates that the A3G rs8177832 polymorphism is associated with a decreased risk of CHB infection and HCC, while the rs2011861 polymorphism is associated with an increased risk of HCC.