Combining the Pharmaceutical and Toxicological Properties of Selected Essential Oils with their Chemical Components by GC‐MS Analysis

• Published in: Chemistry & biodiversity Vol. 21; no. 7; pp. e202400738 - n/a
• Main Authors: Nilofar, Ahmed, Shakeel, Zengin, Gokhan, Di Simone, Simonetta Cristina, Acquaviva, Alessandra, Libero, Maria Loreta, Chiavaroli, Annalisa, Orlando, Giustino, Tacchini, Massimo, Di Vito, Maura, Menghini, Luigi, Ferrante, Claudio
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 01.07.2024
• Subjects: Allelopathy; Anticancer properties; Antioxidant effects; Antioxidants; Antitumor activity; Aromatic plants; Cancer; Chemical composition; Colorectal cancer; Colorectal carcinoma; Cytotoxicity; Enzyme inhibition; Essential oils; Gas chromatography; Gene expression; Glucosidase; Mass spectrometry; Mass spectroscopy; Oils & fats; Oregano; Plant extracts; Plant species; Supply chains; α-Amylase
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202400738

In the current investigation, a comprehensive analysis was carried out on essential oils (EOs) extracted from six aromatic plant species, namely Rosmarinus officinalis, Pelargonium graveolens, Thymus vulgaris, Origanum vulgare, Laurus nobilis, and Aloysia citrodora. An exploration was conducted into the chemical composition using Gas Chromatography‐Mass Spectrometry (GC/MS), antioxidant properties assessed through DPPH, ABTS, CUPRAC, FRAP, MCA, and PBD assays, ecotoxicological impacts evaluated via allelopathy and the Daphnia magna heartbeat test, as well as bio‐pharmacological effects including anticancer activity and gene expression analysis. Results revealed strong antioxidant activity in all essential oils, with T. vulgaris EO (2748.00 mg TE/g) and O. vulgare EO (2609.29 mg TE/g) leading in CUPRAC assay. R. officinalis EO showed the highest α‐amylase inhibition at 1.58 mmol ACAE/g, while O. vulgare EO excelled in α‐glucosidase inhibition at 1.57 mmol ACAE/g. Additionally, cytotoxic effects were evaluated on human colorectal cancer (HCT116) cells. A. citrodora, O. vulgare, and R. officinalis EOs were found the most potent anticancer, as also witnessed by their higher modulatory effects on the gene expression of BAX and Bcl‐2. Collectively, the present data highlight the importance to implement the knowledge and to valorize the supply chain of aromatic plants.