DNA analysis and sorting of rat testis cells using two‐parameter flow cytometry

By use of two‐parameter flow cytometry of rat testis cell suspensions stained with mithramycin for DNA (the peak amplitude of the fluorescence signal versus total fluorescence intensity integrated over time), eight cell compartments could be distinguished without pre‐enrichment of the samples. Cells...

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Published inCytometry (New York, N.Y.) Vol. 6; no. 4; pp. 321 - 326
Main Authors van Kroonenburgh, Marinus J., Beck, Johannes L., Scholtz, Johannes W., Hacker‐Klom, Ursula, Herman, Chester J.
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.1985
Wiley-Liss
Subjects
Animals
Biological and medical sciences
Cell Separation
cell sorting
DNA - analysis
Fibroblasts - analysis
Flow Cytometry - methods
Fundamental and applied biological sciences. Psychology
Leydig Cells - analysis
Male
Mammalian male genital system
Morphology. Physiology
octaploid cells
rat spermatogenesis
Rats
Sertoli Cells - analysis
Spermatids - analysis
Spermatocytes - analysis
Spermatogonia - analysis
Testis - cytology
Two‐parameter flow cytometry
Vertebrates: reproduction
Flow cytometry
Polyploidy
Rat
Spermatogenesis
Rodentia
Cell biology
Germinal cell
Testicle
Male genital system
Sorting
Vertebrata
Mammalia
DNA
Cell
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Summary:By use of two‐parameter flow cytometry of rat testis cell suspensions stained with mithramycin for DNA (the peak amplitude of the fluorescence signal versus total fluorescence intensity integrated over time), eight cell compartments could be distinguished without pre‐enrichment of the samples. Cells in these compartments were identified by sorting and subsequent microscopic examination.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0196-4763
1097-0320
DOI:10.1002/cyto.990060408